Recombinant Full Length Human DNAI1 Protein, C-Flag-tagged

• Cat.No.: DNAI1-2186HFL
• Product Overview: Recombinant Full Length Human DNAI1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 79.1 kDa
• AA Sequence: MIPASAKSPHKQPHKQSISIGRGTR KRDEDSGTEVGEGTDEWAQSKATVR PPDQLELTDAELKEEFTRIL TANNPHAPQNIVRYSFKEGTYKPIG FVNQLAVHYTQVGNLIPKDSDEGRR QHYRDELVAGSQESVKVISE TGNLEEDEEPKELETEPGSQTDVPA AGAAEKVTEEELMTPKQPKERKLTN QFNFSERASQTCNNPVRDRE CQTEPPPRTNFSATANQWEIYDAYV EELEKQEKTKEKEKAKTPVAKKSGK MAMRKLTSMESQTDDLIKLS QAAKIMERMVNQNTYDDIAQDFKYY DDAADEYRDQVGTLLPLWKFQNDKA KRLSVTALCWNPKYRDLFAV GYGSYDFMKQSRGMLLLYSLKNPSF PEYMFSSNSGVMCLDIHVDHPYLVA VGHYDGNVAIYNLKKPHSQP SFCSSAKSGKHSDPVWQVKWQKDDM DQNLNFFSVSSDGRIVSWTLVKRKL VHIDVIKLKVEGSTTEVPEG LQLHQVGCGTAFDFHKEIDYMFLVG TEEGKIYKCSKSYSSQFLDTYDAHN MSVDTVSWNPYHTKVFMSCS SDWTVKIWDHTIKTPMFIYDLNSAV GDVAWAPYSSTVFAAVTTDGKAHIF DLAINKYEAICNQPVAAKKN RLTHVQFNLIHPIIIVGDDRGHIIS LKLSPNLRKMPKEKKGQEVQKGPAV EIAKLDKLLNLVREVKIKT TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease

Gene Information

• Gene Name: DNAI1 dynein axonemal intermediate chain 1 [ Homo sapiens (human) ]
• Official Symbol: DNAI1
• Synonyms: PCD; DIC1; ICS1; CILD1
• Gene ID: 27019
• mRNA Refseq: NM_012144.4
• Protein Refseq: NP_036276.1
• MIM: 604366
• UniProt ID: Q9UI46