Recombinant Full Length Human EIF2A Protein, C-Flag-tagged

• Cat.No.: EIF2A-58HFL
• Product Overview: Recombinant Full Length Human EIF2A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 64.8 kDa
• AA Sequence: MAPSTPLLTVRGSEGLYMVNGPPHF TESTVFPRESGKNCKVCIFSKDGTL FAWGNGEKVNIISVTNKGLL HSFDLLKAVCLEFSPKNTVLATWQP YTTSKDGTAGIPNLQLYDVKTGTCL KSFIQKKMQNWCPSWSEDET LCARNVNNEVHFFENNNFNTIANKL HLQKINDFVLSPGPQPYKVAVYVPG SKGAPSFVRLYQYPNFAGPH AALANKSFFKADKVTMLWNKKATAV LVIASTDVDKTGASYYGEQTLHYIA TNGESAVVQLPKNGPIYDVV WNSSSTEFCAVYGFMPAKATIFNLK CDPVFDFGTGPRNAAYYSPHGHILV LAGFGNLRGQMEVWDVKNYK LISKPVASDSTYFAWCPDGEHILTA TCAPRLRVNNGYKIWHYTGSILHKY DVPSNAELWQVSWQPFLDGI FPAKTITYQAVPSEVPNEEPKVATA YRPPALRNKPITNSKLHEEEPPQNM KPQSGNDKPLSKTALKNQRK HEAKKAAKQEARSDKSPDLAPTPAP QSTPRNTVSQSISGDPEIDKKIKNL KKKLKAIEQLKEQAATGKQL
  EKNQLEKIQKETALLQELEDLKLGI TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: EIF2A eukaryotic translation initiation factor 2A [ Homo sapiens (human) ]
• Official Symbol: EIF2A
• Synonyms: CDA02; EIF-2A; MST089; MSTP004; MSTP089
• Gene ID: 83939
• mRNA Refseq: NM_032025.5
• Protein Refseq: NP_114414.2
• MIM: 609234
• UniProt ID: Q9BY44