Recombinant Full Length Human EIF4A1 Protein, C-Flag-tagged

• Cat.No.: EIF4A1-314HFL
• Product Overview: Recombinant Full Length Human EIF4A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46 kDa
• AA Sequence: MSASQDSRSRDNGPDGMEPEGVIES NWNEIVDSFDDMNLSESLLRGIYAY GFEKPSAIQQRAILPCIKGY DVIAQAQSGTGKTATFAISILQQIE LDLKATQALVLAPTRELAQQIQKVV MALGDYMGASCHACIGGTNV RAEVQKLQMEAPHIIVGTPGRVFDM LNRRYLSPKYIKMFVLDEADEMLSR GFKDQIYDIFQKLNSNTQVV LLSATMPSDVLEVTKKFMRDPIRIL VKKEELTLEGIRQFYINVEREEWKL DTLCDLYETLTITQAVIFIN TRRKVDWLTEKMHARDFTVSAMHGD MDQKERDVIMREFRSGSSRVLITTD LLARGIDVQQVSLVINYDLP
  TNRENYIHRIGRGGRFGRKGVAINM VTEEDKRTLRDIETFYNTSIEEMPL NVADLISGPTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: EIF4A1 eukaryotic translation initiation factor 4A1 [ Homo sapiens (human) ]
• Official Symbol: EIF4A1
• Synonyms: DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
• Gene ID: 1973
• mRNA Refseq: NM_001416.4
• Protein Refseq: NP_001407.1
• MIM: 602641
• UniProt ID: P60842