Recombinant Full Length Human FADD Protein, C-Flag-tagged

• Cat.No.: FADD-1644HFL
• Product Overview: Recombinant Full Length Human FADD Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 23.1 kDa
• AA Sequence: MDPFLVLLHSVSSSLSSSELTELKF LCLGRVGKRKLERVQSGLDLFSMLL EQNDLEPGHTELLRELLASL RRHDLLRRVDDFEAGAAAGAAPGEE DLCAAFNVICDNVGKDWRRLARQLK VSDTKIDSIEDRYPRNLTER VRESLRIWKNTEKENATVAHLVGAL RSCQMNLVADLVQEVQQARDLQNRS GAMSPMSWNSDASTSEASTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Apoptosis, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway

Gene Information

• Gene Name: FADD Fas associated via death domain [ Homo sapiens (human) ]
• Official Symbol: FADD
• Synonyms: GIG3; IMD90; MORT1
• Gene ID: 8772
• mRNA Refseq: NM_003824.4
• Protein Refseq: NP_003815.1
• MIM: 602457
• UniProt ID: Q13158