Recombinant Full Length Human FURIN Protein, C-Flag-tagged

• Cat.No.: FURIN-861HFL
• Product Overview: Recombinant Full Length Human FURIN Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 86.5 kDa
• AA Sequence: MELRPWLLWVVAATGTLVLLAADAQ GQKVFTNTWAVRIPGGPAVANSVAR KHGFLNLGQIFGDYYHFWHR GVTKRSLSPHRPRHSRLQREPQVQW LEQQVAKRRTKRDVYQEPTDPKFPQ QWYLSGVTQRDLNVKAAWAQ GYTGHGIVVSILDDGIEKNHPDLAG NYDPGASFDVNDQDPDPQPRYTQMN DNRHGTRCAGEVAAVANNGV CGVGVAYNARIGGVRMLDGEVTDAV EARSLGLNPNHIHIYSASWGPEDDG KTVDGPARLAEEAFFRGVSQ GRGGLGSIFVWASGNGGREHDSCNC DGYTNSIYTLSISSATQFGNVPWYS EACSSTLATTYSSGNQNEKQ IVTTDLRQKCTESHTGTSASAPLAA GIIALTLEANKNLTWRDMQHLVVQT SKPAHLNANDWATNGVGRKV SHSYGYGLLDAGAMVALAQNWTTVA PQRKCIIDILTEPKDIGKRLEVRKT VTACLGEPNHITRLEHAQAR LTLSYNRRGDLAIHLVSPMGTRSTL LAARPHDYSADGFNDWAFMTTHSWD EDPSGEWVLEIENTSEANNY GTLTKFTLVLYGTAPEGLPVPPESS GCKTLTSSQACVVCEEGFSLHQKSC VQHCPPGFAPQVLDTHYSTE NDVETIRASVCAPCHASCATCQGPA LTDCLSCPSHASLDPVEQTCSRQSQ SSRESPPQQQPPRLPPEVEA GQRLRAGLLPSHLPEVVAGLSCAFI VLVFVTVFLVLQLRSGFSFRGVKVY TMDRGLISYKGLPPEAWQEE
  CPSDSEEDEGRGERTAFIKDQSALT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease, Transmembrane

Gene Information

• Gene Name: FURIN furin, paired basic amino acid cleaving enzyme [ Homo sapiens (human) ]
• Official Symbol: FURIN
• Synonyms: FUR; PACE; SPC1; PCSK3
• Gene ID: 5045
• mRNA Refseq: NM_002569.4
• Protein Refseq: NP_002560.1
• MIM: 136950
• UniProt ID: P09958