| Description : |
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
86.7 kDa |
| AA Sequence : |
MELRPWLLWVVAATGTLVLLAADAQ GQKVFTNTWAVRIPGGPAVANSVAR KHGFLNLGQIFGDYYHFWHRGVTKR SLSPHRPRHSRLQREPQVQWLEQQV AKRRTKRDVYQEPTDPKFPQQWYLS GVTQRDLNVKAAWAQGYTGHGIVVS ILDDGIEKNHPDLAGNYDPGASFDV NDQDPDPQPRYTQMNDNRHGTRCAG EVAAVANNGVCGVGVAYNARIGGVR MLDGEVTDAVEARSLGLNPNHIHIY SASWGPEDDGKTVDGPARLAEEAFF RGVSQGRGGLGSIFVWASGNGGREH DSCNCDGYTNSIYTLSISSATQFGN VPWYSEACSSTLATTYSSGNQNEKQ IVTTDLRQKCTESHTGTSASAPLAA GIIALTLEANKNLTWRDMQHLVVQT SKPAHLNANDWATNGVGRKVSHSYG YGLLDAGAMVALAQNWTTVAPQRKC IIDILTEPKDIGKRLEVRKTVTACL GEPNHITRLEHAQARLTLSYNRRGD LAIHLVSPMGTRSTLLAARPHDYSA DGFNDWAFMTTHSWDEDPSGEWVLE IENTSEANNYGTLTKFTLVLYGTAP EGLPVPPESSGCKTLTSSQACVVCE EGFSLHQKSCVQHCPPGFAPQVLDT HYSTENDVETIRASVCAPCHASCAT CQGPALTDCLSCPSHASLDPVEQTC SRQSQSSRESPPQQQPPRLPPEVEA GQRLRAGLLPSHLPEVVAGLSCAFI VLVFVTVFLVLQLRSGFSFRGVKVY TMDRGLISYKGLPPEAWQEECPSDS EEDEGRGERTAFIKDQSALTRTRPL EQKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
