Recombinant Full Length Human HAO1 Protein, C-Flag-tagged

• Cat.No.: HAO1-1303HFL
• Product Overview: Recombinant Full Length Human HAO1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 40.7 kDa
• AA Sequence: MLPRLICINDYEQHAKSVLPKSIYD YYRSGANDEETLADNIAAFSRWKLY PRMLRNVAETDLSTSVLGQR VSMPICVGATAMQRMAHVDGELATV RACQSLGTGMMLSSWATSSIEEVAE AGPEALRWLQLYIYKDREVT KKLVRQAEKMGYKAIFVTVDTPYLG NRLDDVRNRFKLPPQLRMKNFETST LSFSPEENFGDDSGLAAYVA KAIDPSISWEDIKWLRRLTSLPIVA KGILRGDDAREAVKHGLNGILVSNH GARQLDGVPATIDVLPEIVE AVEGKVEVFLDGGVRKGTDVLKALA LGAKAVFVGRPIVWGLAFQGEKGVQ DVLEILKEEFRLAMALSGCQ
  NVKVIDKTLVRKNPLAVSKITRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways

Gene Information

• Gene Name: HAO1 hydroxyacid oxidase 1 [ Homo sapiens (human) ]
• Official Symbol: HAO1
• Synonyms: GO; GOX; GOX1; HAOX1
• Gene ID: 54363
• mRNA Refseq: NM_017545.3
• Protein Refseq: NP_060015.1
• MIM: 605023
• UniProt ID: Q9UJM8