Recombinant Full Length Human HBA2 Protein, C-Flag-tagged

• Cat.No.: HBA2-856HFL
• Product Overview: Recombinant Full Length Human HBA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 15.1 kDa
• AA Sequence: MVLSPADKTNVKAAWGKVGAHAGEY GAEALERMFLSFPTTKTYFPHFDLS HGSAQVKGHGKKVADALTNA VAHVDDMPNALSALSDLHAHKLRVD PVNFKLLSHCLLVTLAAHLPAEFTP AVHASLDKFLASVSTVLTSK
  YRTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: HBA2 hemoglobin subunit alpha 2 [ Homo sapiens (human) ]
• Official Symbol: HBA2
• Synonyms: HBH; ECYT7; HBA-T2
• Gene ID: 3040
• mRNA Refseq: NM_000517.6
• Protein Refseq: NP_000508.1
• MIM: 141850
• UniProt ID: P69905