Recombinant Full Length Human HBB Protein, C-Flag-tagged

• Cat.No.: HBB-181HFL
• Product Overview: Recombinant Full Length Human HBB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 15.8 kDa
• AA Sequence: MVHLTPEEKSAVTALWGKVNVDEVG GEALGRLLVVYPWTQRFFESFGDLS TPDAVMGNPKVKAHGKKVLG AFSDGLAHLDNLKGTFATLSELHCD KLHVDPENFRLLGNVLVCVLAHHFG KEFTPPVQAAYQKVVAGVAN
  ALAHKYHTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: HBB hemoglobin subunit beta [ Homo sapiens (human) ]
• Official Symbol: HBB
• Synonyms: ECYT6; CD113t-C; beta-globin
• Gene ID: 3043
• mRNA Refseq: NM_000518.5
• Protein Refseq: NP_000509.1
• MIM: 141900
• UniProt ID: P68871