Recombinant Full Length Human HPRT1 Protein, C-Flag-tagged

• Cat.No.: HPRT1-619HFL
• Product Overview: Recombinant Full Length Human HPRT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 24.4 kDa
• AA Sequence: MATRSPGVVISDDEPGYDLDLFCIP NHYAEDLERVFIPHGLIMDRTERLA RDVMKEMGGHHIVALCVLKG GYKFFADLLDYIKALNRNSDRSIPM TVDFIRLKSYCNDQSTGDIKVIGGD DLSTLTGKNVLIVEDIIDTG KTMQTLLSLVRQYNPKMVKVASLLV KRTPRSVGYKPDFVGFEIPDKFVVG YALDYNEYFRDLNHVCVISE
  TGKAKYKATRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism

Gene Information

• Gene Name: HPRT1 hypoxanthine phosphoribosyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: HPRT1
• Synonyms: HPRT; HGPRT
• Gene ID: 3251
• mRNA Refseq: NM_000194.3
• Protein Refseq: NP_000185.1
• MIM: 308000
• UniProt ID: P00492