Recombinant Full Length Human IDE Protein, C-Flag-tagged

• Cat.No.: IDE-307HFL
• Product Overview: Recombinant Full Length Human IDE Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 117.8 kDa
• AA Sequence: MRYRLAWLLHPALPSTFRSVLGARL PPPERLCGFQKKTYSKMNNPAIKRI GNHITKSPEDKREYRGLELA NGIKVLLMSDPTTDKSSAALDVHIG SLSDPPNIAGLSHFCEHMLFLGTKK YPKENEYSQFLSEHAGSSNA FTSGEHTNYYFDVSHEHLEGALDRF AQFFLCPLFDESCKDREVNAVDSEH EKNVMNDAWRLFQLEKATGN PKHPFSKFGTGNKYTLETRPNQEGI DVRQELLKFHSAYYSSNLMAVCVLG RESLDDLTNLVVKLFSEVEN KNVPLPEFPEHPFQEEHLKQLYKIV PIKDIRNLYVTFPIPDLQKYYKSNP GHYLGHLIGHEGPGSLLSEL KSKGWVNTLVGGQKEGARGFMFFII NVDLTEEGLLHVEDIILHMFQYIQK LRAEGPQEWVFQECKDLNAV AFRFKDKERPRGYTSKIAGILHYYP LEEVLTAEYLLEEFRPDLIEMVLDK LRPENVRVAIVSKSFEGKTD RTEEWYGTQYKQEAIPDEVIKKWQN ADLNGKFKLPTKNEFIPTNFEILPL EKEATPYPALIKDTVMSKLW FKQDDKKKKPKACLNFEFFSPFAYV DPLHCNMAYLYLELLKDSLNEYAYA AELAGLSYDLQNTIYGMYLS VKGYNDKQPILLKKIIEKMATFEID EKRFEIIKEAYMRSLNNFRAEQPHQ HAMYYLRLLMTEVAWTKDEL KEALDDVTLPRLKAFIPQLLSRLHI EALLHGNITKQAALGIMQMVEDTLI EHAHTKPLLPSQLVRYREVQ LPDRGWFVYQQRNEVHNNCGIEIYY QTDMQSTSENMFLELFCQIISEPCF NTLRTKEQLGYIVFSGPRRA NGIQSLRFIIQSEKPPHYLESRVEA FLITMEKSIEDMTEEAFQKHIQALA IRRLDKPKKLSAECAKYWGE IISQQYNFDRDNTEVAYLKTLTKED IIKFYKEMLAVDAPRRHKVSVHVLA REMDSCPVVGEFPCQNDINL
  SQAPALPQPEVIQNMTEFKRGLPLF PLVKPHINFMAAKLTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease

Gene Information

• Gene Name: IDE insulin degrading enzyme [ Homo sapiens (human) ]
• Official Symbol: IDE
• Synonyms: INSULYSIN
• Gene ID: 3416
• mRNA Refseq: NM_004969.4
• Protein Refseq: NP_004960.2
• MIM: 146680
• UniProt ID: P14735