Recombinant Full Length Human IDH3A Protein, C-Flag-tagged

• Cat.No.: IDH3A-1349HFL
• Product Overview: Recombinant Full Length Human IDH3A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 36.6 kDa
• AA Sequence: MAGPAWISKVSRLLGAFHNPKQVTR GFTGGVQTVTLIPGDGIGPEISAAV MKIFDAAKAPIQWEERNVTA IQGPGGKWMIPSEAKESMDKNKMGL KGPLKTPIAAGHPSMNLLLRKTFDL YANVRPCVSIEGYKTPYTDV NIVTIRENTEGEYSGIEHVIVDGVV QSIKLITEGASKRIAEFAFEYARNN HRSNVTAVHKANIMRMSDGL FLQKCREVAESCKDIKFNEMYLDTV CLNMVQDPSQFDVLVMPNLYGDILS DLCAGLIGGLGVTPSGNIGA NGVAIFESVHGTAPDIAGKDMANPT ALLLSAVMMLRHMGLFDHAARIEAA CFATIKDGKSLTKDLGGNAK
  CSDFTEEICRRVKDLDTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways

Gene Information

• Gene Name: IDH3A isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha [ Homo sapiens (human) ]
• Official Symbol: IDH3A
• Synonyms: RP90
• Gene ID: 3419
• mRNA Refseq: NM_005530.3
• Protein Refseq: NP_005521.1
• MIM: 601149
• UniProt ID: P50213