Recombinant Human IDH3A Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: IDH3A-2376H
• Product Overview: IDH3A MS Standard C13 and N15-labeled recombinant protein (NP_005521) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 39.6 kDa
• AA Sequence: MAGPAWISKVSRLLGAFHNPKQVTR GFTGGVQTVTLIPGDGIGPEISAAV MKIFDAAKAPIQWEERNVTAIQGPG GKWMIPSEAKESMDKNKMGLKGPLK TPIAAGHPSMNLLLRKTFDLYANVR PCVSIEGYKTPYTDVNIVTIRENTE GEYSGIEHVIVDGVVQSIKLITEGA SKRIAEFAFEYARNNHRSNVTAVHK ANIMRMSDGLFLQKCREVAESCKDI KFNEMYLDTVCLNMVQDPSQFDVLV MPNLYGDILSDLCAGLIGGLGVTPS GNIGANGVAIFESVHGTAPDIAGKD MANPTALLLSAVMMLRHMGLFDHAA RIEAACFATIKDGKSLTKDLGGNAK CSDFTEEICRRVKDLDTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: IDH3A isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha [ Homo sapiens (human) ]
• Official Symbol: IDH3A
• Synonyms: IDH3A; isocitrate dehydrogenase 3 (NAD+) alpha; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; H IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subunit alpha; mitochondrial; isocitric dehydrogenase; NAD(H) specific isocitrate dehydrogenase alpha subunit; NAD+ specific ICDH; H-IDH alpha; NAD+-specific ICDH; NAD(+)-specific ICDH subunit alpha; isocitric dehydrogenase subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit
• Gene ID: 3419
• mRNA Refseq: NM_005530
• Protein Refseq: NP_005521
• MIM: 601149
• UniProt ID: P50213