| Description : |
This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Deficiencies in the encoded protein, SHIP1, have been associated with Inflammatory Bowel Disease types such as Crohn's Disease and Ulcerative Colitis. Alternative splicing of this gene results in multiple transcript variants. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Molecular Mass : |
133 kDa |
| AA Sequence : |
MVPCWNHGNITRSKAEELLSRTGKD GSFLVRASESISRAYALCVLYRNCV YTYRILPNEDDKFTVQASEG VSMRFFTKLDQLIEFYKKENMGLVT HLQYPVPLEEEDTGDDPEEDTESVV SPPELPPRNIPLTASSCEAK EVPFSNENPRATETSRPSLSETLFQ RLQSMDTSGLPEEHLKAIQDYLSTQ LAQDSEFVKTGSSSLPHLKK LTTLLCKELYGEVIRTLPSLESLQR LFDQQLSPGLRPRPQVPGEANPINM VSKLSQLTSLLSSIEDKVKA LLHEGPESPHRPSLIPPVTFEVKAE SLGIPQKMQLKVDVESGKLIIKKSK DGSEDKFYSHKKILQLIKSQ KFLNKLVILVETEKEKILRKEYVFA DSKKREGFCQLLQQMKNKHSEQPEP DMITIFIGTWNMGNAPPPKK ITSWFLSKGQGKTRDDSADYIPHDI YVIGTQEDPLSEKEWLEILKHSLQE ITSVTFKTVAIHTLWNIRIV VLAKPEHENRISHICTDNVKTGIAN TLGNKGAVGVSFMFNGTSLGFVNSH LTSGSEKKLRRNQNYMNILR FLALGDKKLSPFNITHRFTHLFWFG DLNYRVDLPTWEAETIIQKIKQQQY ADLLSHDQLLTERREQKVFL HFEEEEITFAPTYRFERLTRDKYAY TKQKATGMKYNLPSWCDRVLWKSYP LVHVVCQSYGSTSDIMTSDH SPVFATFEAGVTSQFVSKNGPGTVD SQGQIEFLRCYATLKTKSQTKFYLE FHSSCLESFVKSQEGENEEG SEGELVVKFGETLPKLKPIISDPEY LLDQHILISIKSSDSDESYGEGCIA LRLEATETQLPIYTPLTHHG ELTGHFQGEIKLQTSQGKTREKLYD FVKTERDESSGPKTLKSLTSHDPMK QWEVTSRAPPCSGSSITEII NPNYMGVGPFGPPMPLHVKQTLSPD QQPTAWSYDQPPKDSPLGPCRGESP PTPPGQPPISPKKFLPSTAN RGLPPRTQESRPSDLGKNAGDTLPQ EDLPLTKPEMFENPLYGSLSSFPKP APRKDQESPKMPRKEPPPCP EPGILSPSIVLTKAQEADRGEGPGK QVPAPRLRSFTCSSSAEGRAAGGDK SQGKPKTPVSSQAPVPAKRP IKPSRSEINQQTPPTPTPRPPLPVK SPAVLHLQHSKGRDYRDNTELPHHG KHRPEEGPPGPLGRTAMQTRTRPLE QKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome |
| Protein Pathways : |
B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, Phosphatidylinositol signaling system |
