Recombinant Full Length Human ITGAM Protein, C-Flag-tagged

• Cat.No.: ITGAM-737HFL
• Product Overview: Recombinant Full Length Human ITGAM Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 125.4 kDa
• AA Sequence: MALRVLLLTALTLCHGFNLDTENAM TFQENARGFGQSVVQLQGSRVVVGA PQEIVAANQRGSLYQCDYST GSCEPIRLQVPVEAVNMSLGLSLAA TTSPPQLLACGPTVHQTCSENTYVK GLCFLFGSNLRQQPQKFPEA LRGCPQEDSDIAFLIDGSGSIIPHD FRRMKEFVSTVMEQLKKSKTLFSLM QYSEEFRIHFTFKEFQNNPN PRSLVKPITQLLGRTHTATGIRKVV RELFNITNGARKNAFKILVVITDGE KFGDPLGYEDVIPEADREGV IRYVIGVGDAFRSEKSRQELNTIAS KPPRDHVFQVNNFEALKTIQNQLRE KIFAIEGTQTGSSSSFEHEM SQEGFSAAITSNGPLLSTVGSYDWA GGVFLYTSKEKSTFINMTRVDSDMN DAYLGYAAAIILRNRVQSLV LGAPRYQHIGLVAMFRQNTGMWESN ANVKGTQIGAYFGASLCSVDVDSNG STDLVLIGAPHYYEQTRGGQ VSVCPLPRGRARWQCDAVLYGEQGQ PWGRFGAALTVLGDVNGDKLTDVAI GAPGEEDNRGAVYLFHGTSG SGISPSHSQRIAGSKLSPRLQYFGQ SLSGGQDLTMDGLVDLTVGAQGHVL LLRSQPVLRVKAIMEFNPRE VARNVFECNDQVVKGKEAGEVRVCL HVQKSTRDRLREGQIQSVVTYDLAL DSGRPHSRAVFNETKNSTRR QTQVLGLTQTCETLKLQLPNCIEDP VSPIVLRLNFSLVGTPLSAFGNLRP VLAEDAQRLFTALFPFEKNC GNDNICQDDLSITFSFMSLDCLVVG GPREFNVTVTVRNDGEDSYRTQVTF FFPLDLSYRKVSTLQNQRSQ RSWRLACESASSTEVSGALKSTSCS INHPIFPENSEVTFNITFDVDSKAS LGNKLLLKANVTSENNMPRT NKTEFQLELPVKYAVYMVVTSHGVS TKYLNFTASENTSRVMQHQYQVSNL GQRSLPISLVFLVPVRLNQT VIWDRPQVTFSENLSSTCHTKERLP SHSDFLAELRKAPVVNCSIAVCQRI QCDIPFFGIQEEFNATLKGN LSFDWYIKTSHNHLLIVSTAEILFN DSVFTLLPGQGAFVRSQTETKVEPF EVPNPLPLIVGSSVGGLLLL
  ALITAALYKLGFFKRQYKDMMSEGG PPGAEPQTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

Gene Information

• Gene Name: ITGAM integrin subunit alpha M [ Homo sapiens (human) ]
• Official Symbol: ITGAM
• Synonyms: CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• Gene ID: 3684
• mRNA Refseq: NM_000632.4
• Protein Refseq: NP_000623.2
• MIM: 120980
• UniProt ID: P11215