Recombinant Full Length Human JAK2 Protein, C-Flag-tagged

• Cat.No.: JAK2-177HFL
• Product Overview: Recombinant Full Length Human JAK2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 130.5 kDa
• AA Sequence: MGMACLTMTEMEGTSTSSIYQNGDI SGNANSMKQIDPVLQVYLYHSLGKS EADYLTFPSGEYVAEEICIA ASKACGITPVYHNMFALMSETERIW YPPNHVFHIDESTRHNVLYRIRFYF PRWYCSGSNRAYRHGISRGA EAPLLDDFVMSYLFAQWRHDFVHGW IKVPVTHETQEECLGMAVLDMMRIA KENDQTPLAIYNSISYKTFL PKCIRAKIQDYHILTRKRIRYRFRR FIQQFSQCKATARNLKLKYLINLET LQSAFYTEKFEVKEPGSGPS GEEIFATIIITGNGGIQWSRGKHKE SETLTEQDLQLYCDFPNIIDVSIKQ ANQEGSNESRVVTIHKQDGK NLEIELSSLREALSFVSLIDGYYRL TADAHHYLCKEVAPPAVLENIQSNC HGPISMDFAISKLKKAGNQT GLYVLRCSPKDFNKYFLTFAVEREN VIEYKHCLITKNENEEYNLSGTKKN FSSLKDLLNCYQMETVRSDN IIFQFTKCCPPKPKDKSNLLVFRTN GVSDVPTSPTLQRPTHMNQMVFHKI RNEDLIFNESLGQGTFTKIF KGVRREVGDYGQLHETEVLLKVLDK AHRNYSESFFEAASMMSKLSHKHLV LNYGVCVCGDENILVQEFVK FGSLDTYLKKNKNCINILWKLEVAK QLAWAMHFLEENTLIHGNVCAKNIL LIREEDRKTGNPPFIKLSDP GISITVLPKDILQERIPWVPPECIE NPKNLNLATDKWSFGTTLWEICSGG DKPLSALDSQRKLQFYEDRH QLPAPKWAELANLINNCMDYEPDFR PSFRAIIRDLNSLFTPDYELLTEND MLPNMRIGALGFSGAFEDRD PTQFEERHLKFLQQLGKGNFGSVEM CRYDPLQDNTGEVVAVKKLQHSTEE HLRDFEREIEILKSLQHDNI VKYKGVCYSAGRRNLKLIMEYLPYG SLRDYLQKHKERIDHIKLLQYTSQI CKGMEYLGTKRYIHRDLATR NILVENENRVKIGDFGLTKVLPQDK EYYKVKEPGESPIFWYAPESLTESK FSVASDVWSFGVVLYELFTY IEKSKSPPAEFMRMIGNDKQGQMIV FHLIELLKNNGRLPRPDGCPDEIYM IMTECWNNNVNQRPSFRDLA
  LRVDQIRDNMAGTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway

Gene Information

• Gene Name: JAK2 Janus kinase 2 [ Homo sapiens (human) ]
• Official Symbol: JAK2
• Synonyms: JTK10
• Gene ID: 3717
• mRNA Refseq: NM_004972.4
• Protein Refseq: NP_004963.1
• MIM: 147796
• UniProt ID: O60674