Recombinant Full Length Human LAMC2 Protein, C-Flag-tagged

• Cat.No.: LAMC2-1564HFL
• Product Overview: Recombinant Full Length Human LAMC2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 128.7 kDa
• AA Sequence: MPALWLGCCLCFSLLLPAARATSRR EVCDCNGKSRQCIFDRELHRQTGNG FRCLNCNDNTDGIHCEKCKN GFYRHRERDRCLPCNCNSKGSLSAR CDNSGRCSCKPGVTGARCDRCLPGF HMLTDAGCTQDQRLLDSKCD CDPAGIAGPCDAGRCVCKPAVTGER CDRCRSGYYNLDGGNPEGCTQCFCY GHSASCRSSAEYSVHKITST FHQDVDGWKAVQRNGSPAKLQWSQR HQDVFSSAQRLDPVYFVAPAKFLGN QQVSYGQSLSFDYRVDRGGR HPSAHDVILEGAGLRITAPLMPLGK TLPCGLTKTYTFRLNEHPSNNWSPQ LSYFEYRRLLRNLTALRIRA TYGEYSTGYIDNVTLISARPVSGAP APWVEQCICPVGYKGQFCQDCASGY KRDSARLGPFGTCIPCNCQG GGACDPDTGDCYSGDENPDIECADC PIGFYNDPHDPRSCKPCPCHNGFSC SVMPETEEVVCNNCPPGVTG ARCELCADGYFGDPFGEHGPVRPCQ PCQCNNNVDPSASGNCDRLTGRCLK CIHNTAGIYCDQCKAGYFGD PLAPNPADKCRACNCNPMGSEPVGC RSDGTCVCKPGFGGPNCEHGAFSCP ACYNQVKIQMDQFMQQLQRM EALISKAQGGDGVVPDTELEGRMQQ AEQALQDILRDAQISEGASRSLGLQ LAKVRSQENSYQSRLDDLKM TVERVRALGSQYQNRVRDTHRLITQ MQLSLAESEASLGNTNIPASDHYVG PNGFKSLAQEATRLAESHVE SASNMEQLTRETEDYSKQALSLVRK ALHEGVGSGSGSPDGAVVQGLVEKL EKTKSLAQQLTREATQAEIE ADRSYQHSLRLLDSVSRLQGVSDQS FQVEEAKRIKQKADSLSTLVTRHMD EFKRTQKNLGNWKEEAQQLL QNGKSGREKSDQLLSRANLAKSRAQ EALSMGNATFYEVESILKNLREFDL QVDNRKAEAEEAMKRLSYIS QKVSDASDKTQQAERALGSAAADAQ RAKNGAGEALEISSEIEQEIGSLNL EANVTADGALAMEKGLASLK SEMREVEGELERKELEFDTNMDAVQ MVITEAQKVDTRAKNAGVTIQDTLN TLDGLLHLMDQPLSVDEEGL VLLEQKLSRAKTQINSQLRPMMSEL EERARQQRGHLHLLETSIDGILADV KNLENIRDNLPPGCYNTQAL
  EQQTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer

Gene Information

• Gene Name: LAMC2 laminin subunit gamma 2 [ Homo sapiens (human) ]
• Official Symbol: LAMC2
• Synonyms: B2T; CSF; EBR2; BM600; EBR2A; JEB3A; JEB3B; LAMB2T; LAMNB2
• Gene ID: 3918
• mRNA Refseq: NM_005562.3
• Protein Refseq: NP_005553.2
• MIM: 150292
• UniProt ID: Q13753