Recombinant Full Length Human LCTL Protein, C-Flag-tagged

• Cat.No.: LCTL-1508HFL
• Product Overview: Recombinant Full Length Human LCTL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of family 1 glycosidases. Glycosidases are enzymes that hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. Most members of family 1 have two conserved glutamic acid residues, which are required for enzymatic activity. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail. It lacks one of the conserved glutamic acid residues important for catalysis, and its function remains to be determined. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 64.9 kDa
• AA Sequence: MKPVWVATLLWMLLLVPRLGAARKG SPEEASFYYGTFPLGFSWGVGSSAY QTEGAWDQDGKGPSIWDVFT HSGKGKVLGNETADVACDGYYKVQE DIILLRELHVNHYRFSLSWPRLLPT GIRAEQVNKKGIEFYSDLID ALLSSNITPIVTLHHWDLPQLLQVK YGGWQNVSMANYFRDYANLCFEAFG DRVKHWITFSDPRAMAEKGY ETGHHAPGLKLRGTGLYKAAHHIIK AHAKAWHSYNTTWRSKQQGLVGISL NCDWGEPVDISNPKDLEAAE RYLQFCLGWFANPIYAGDYPQVMKD YIGRKSAEQGLEMSRLPVFSLQEKS YIKGTSDFLGLGHFTTRYIT ERNYPSRQGPSYQNDRDLIELVDPN WPDLGSKWLYSVPWGFRRLLNFAQT QYGDPPIYVMENGASQKFHC TQLCDEWRIQYLKGYINEMLKAIKD GANIKGYTSWSLLDKFEWEKGYSDR YGFYYVEFNDRNKPRYPKAS VQYYKKIIIANGFPNPREVESWYLK ALETCSINNQMLAAEPLLSHMQMVT EIVVPTVCSLCVLITAVLLM
  LLLRRQSTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane

Gene Information

• Gene Name: LCTL lactase like [ Homo sapiens (human) ]
• Official Symbol: LCTL
• Synonyms: KLG; KLPH
• Gene ID: 197021
• mRNA Refseq: NM_207338.4
• Protein Refseq: NP_997221.2
• MIM: 617060
• UniProt ID: Q6UWM7