Recombinant Full Length Human LEO1 Protein, C-Flag-tagged

• Cat.No.: LEO1-2081HFL
• Product Overview: Recombinant Full Length Human LEO1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 75.2 kDa
• AA Sequence: MADMEDLFGSDADSEAERKDSDSGS DSDSDQENAASGSNASGSESDQDER GDSGQPSNKELFGDDSEDEG ASHHSGSDNHSERSDNRSEASERSD HEDNDPSDVDQHSGSEAPNDDEDEG HRSDGGSHHSEAEGSEKAHS DDEKWGREDKSDQSDDEKIQNSDDE ERAQGSDEDKLQNSDDDEKMQNTDD EERPQLSDDERQQLSEEEKA NSDDERPVASDNDDEKQNSDDEEQP QLSDEEKMQNSDDERPQASDEEHRH SDDEEEQDHKSESARGSDSE DEVLRMKRKNAIASDSEADSDTEVP KDNSGTMDLFGGADDISSGSDGEDK PPTPGQPVDENGLPQDQQEE EPIPETRIEVEIPKVNTDLGNDLYF VKLPNFLSVEPRPFDPQYYEDEFED EEMLDEEGRTRLKLKVENTI RWRIRRDEEGNEIKESNARIVKWSD GSMSLHLGNEVFDVYKAPLQGDHNH LFIRQGTGLQGQAVFKTKLT FRPHSTDSATHRKMTLSLADRCSKT QKIRILPMAGRDPECQRTEMIKKEE ERLRASIRRESQQRRMREKQ HQRGLSASYLEPDRYDEEEEGEESI SLAAIKNRYKGGIREERARIYSSDS DEGSEEDKAQRLLKAKKLTS DEEGEPSGKRKAEDDDKANKKHKKY VISDEEEEDDD TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: LEO1 LEO1 homolog, Paf1/RNA polymerase II complex component [ Homo sapiens (human) ]
• Official Symbol: LEO1
• Synonyms: RDL
• Gene ID: 123169
• mRNA Refseq: NM_138792.4
• Protein Refseq: NP_620147.1
• MIM: 610507
• UniProt ID: Q8WVC0