Recombinant Full Length Human MAP2 Protein, C-Flag-tagged

• Cat.No.: MAP2-214HFL
• Product Overview: Recombinant Full Length Human MAP2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 199.3 kDa
• AA Sequence: MADERKDEAKAPHWTSAPLTEASAH SHPPEIKDQGGAGEGLVRSANGFPY REDEEGAFGEHGSQGTYSNT KENGINGELTSADRETAEEVSARIV QVVTAEAVAVLKGEQEKEAQHKDQT AALPLAAEETANLPPSPPPS PASEQTVTVEEDLLTASKMEFHDQQ ELTPSTAEPSDQKEKESEKQSKPGE DLKHAALVSQPETTKTYPDK KDMQGTEEEKAPLALFGHTLVASLE DMKQKTEPSLVVPGIDLPKEPPTPK EQKDWFIEMPTEAKKDEWGL VAPISPGPLTPMREKDVFDDIPKWE GKQFDSPMPSPFQGGSFTLPLDVMK NEIVTETSPFAPAFLQPDDK KSLQQTSGPATAKDSFKIEEPHEAK PDKMAEAPPSEAMTLPKDAHIPVVE EHVMGKVLEEEKEAINQETV QQRDTFTPSGQEPILTEKETELKLE EKTTISDKEAVPKESKPPKPADEEI GIIQTSTEHTFSEQKDQEPT TDMLKQDSFPVSLEQAVTDSAMTSK TLEKAMTEPSALIEKSSIQELFEMR VDDKDKIEGVGAATSAELDM PFYEDKSGMSKYFETSALKEEATKS IEPGSDYYELSDTRESVHESIDTMS PMHKNGDKEFQTGKESQPSP PAQEAGYSTLAQSYPSDLPEEPSSP QERMFTIDPKVYGEKRDLHSKNKDD LTLSRSLGLGGRSAIEQRSM SINLPMSCLDSIALGFNFGRGHDLS PLASDILTNTSGSMDEGDDYLPATT PALEKAPCFPVESKEEEQIE KVKATGEESTQAEISCESPFLAKDF YKNGTVMAPDLPEMLDLAGTRSRLA SVSADAEVARRKSVPSETVV EDSRTGLPPVTDENHVIVKTDSQLE DLGYCVFNKYTVPLPSPVQDSENLS GESGTFYEGTDDKVRRDLAT DLSLIEVKLAAAGRVKDEFSVDKEA SAHISGDKSGLSKEFDQEKKANDRL DTVLEKSEEHADSKEHAKKT EEAGDEIETFGLGVTYEQALAKDLS IPTDASSEKAEKGLSSVPEIAEVEP SKKVEQGLDFAVQGQLDVKI SDFGQMASGLNIDDRRATELKLEAT QDMTPSSKAPQEADAFMGVESGHMK EGTKVSETEVKQKVAKPDLV HQEAVDKEESYESSGEHESLTMESL KADEGKKETSPESSLIQDEIAVKLS VEIPCPPAVSEADLATDERA DVQMEFIQGPKEESKETPDISITPS DVAEPLHETIVSEPAEIQSEEEEIE AQGEYDKLLFRSDTLQITDL GVSGAREEFVETCPSEHKGVIESVV TIEDDFITVVQTTTDEGESGSHSVR FAALEQPEVERRPSPHDEEE FEVEEAAEAQAEPKDGSPEAPASPE REEVALSEYKTETYDDYKDETTIDD SIMDADSLWVDTQDDDRSIM TEQLETIPKEEKAEKEARRSSLEKH RKEKPFKTGRGRISTPERKVAKKEP STVSRDEVRRKKAVYKKAEL AKKTEVQAHSPSRKFILKPAIKYTR PTHLSCVKRKTTAAGGESALAPSVF KQAKDKVSDGVTKSPEKRSS LPRPSSILPPRRGVSGDRDENSFSL NSSISSSARRTTRSEPIRRAGKSGT STPTTPGSTAITPGTPPSYS SRTPGTPGTPSYPRTPHTPGTPKSA ILVPSEKKVAIIRTPPKSPATPKQL RLINQPLPDLKNVKSKIGST DNIKYQPKGGQVQIVTKKIDLSHVT SKCGSLKNIGHRPGGGRVKIESVKL DFKEKAQAKVGSLDNAHHVP GGGNVKIDSQKLNFREHAKARVDHG AEIITQSPGRSSVASPRRLSNVSSS GSINLLESPQLATLAEDVTA
  ALAKQGLTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS

Gene Information

• Gene Name: MAP2 microtubule associated protein 2 [ Homo sapiens (human) ]
• Official Symbol: MAP2
• Synonyms: MAP-2; MAP2A; MAP2B; MAP2C
• Gene ID: 4133
• mRNA Refseq: NM_002374.4
• Protein Refseq: NP_002365.3
• MIM: 157130
• UniProt ID: P11137