| Description : |
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Molecular Mass : |
44.2 kDa |
| AA Sequence : |
MLARRKPVLPALTINPTIAEGPSPT SEGASEANLVDLQKKLEELELDEQQ KKRLEAFLTQKAKVGELKDD DFERISELGAGNGGVVTKVQHRPSG LIMARKLIHLEIKPAIRNQIIRELQ VLHECNSPYIVGFYGAFYSD GEISICMEHMDGGSLDQVLKEAKRI PEEILGKVSIAVLRGLAYLREKHQI MHRDVKPSNILVNSRGEIKL CDFGVSGQLIDSMANSFVGTRSYMA PERLQGTHYSVQSDIWSMGLSLVEL AVGRYPIPPPDAKELEAIFG RPVVDGEEGEPHSISPRPRPPGRPV SGHGMDSRPAMAIFELLDYIVNEPP PKLPNGVFTPDFQEFVNKCL
IKNPAERADLKMLTNHTFIKRSEVE EVDFAGWLCKTLRLNQPGTPTRTAV TRTRPLEQKLISEEDLAANDILDYK DDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome, Protein Kinase |
| Protein Pathways : |
Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chronic myeloid leukemia, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prion diseases, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway |
