Active Recombinant Full Length Human MAP2K4 Protein, GST-tagged

• Cat.No.: MAP2K4-5287HF
• Product Overview: Full-length human MAP2K4 [1-399(end) amino acids of accession number NP_003001.1] was co- expressed as N-terminal GST-fusion protein (71 kDa) with human His-tagged MAP3K3 [1-626(end) amino acids of accession number NP_002392.2] using baculovirus expression system. GST-MAP2K4 was purified by using glutathione sepharose chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-399
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .
• Purity: 66%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase kinase 4
• Official Symbol: MAP2K4
• Synonyms: MAP2K4;JNKK;JNKK1;MAPKK4;MEK4;MKK4;PRKMK4;SAPKK-1;SAPKK1;SEK1;SERK1;SKK1
• Protein Refseq: NP_003001.1
• UniProt ID: P45985