Recombinant Full Length Human MAP2K4 Protein, GST-tagged MAP2K4-5287HF

Recombinant Full Length Human MAP2K4 Protein, GST-tagged

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Cat.No. : MAP2K4-5287HF Optional Service: Optional requirements on this protein
Product Overview : Full-length human MAP2K4 [1-399(end) amino acids of accession number NP_003001.1] was co- expressed as N-terminal GST-fusion protein (71 kDa) with human His-tagged MAP3K3 [1-626(end) amino acids of accession number NP_002392.2] using baculovirus expression system. GST-MAP2K4 was purified by using glutathione sepharose chromatography.
Source : Insect (sf21)
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .
Protein length : 1-399
Purity : 66%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
SDS-PAGE :

The purity was assessed by SDS-PAGE/CBB staining.

Activity data :

The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .

Gene Name : mitogen-activated protein kinase kinase 4
Official Symbol : MAP2K4
Synonyms : MAP2K4;JNKK;JNKK1;MAPKK4;MEK4;MKK4;PRKMK4;SAPKK-1;SAPKK1;SEK1;SERK1;SKK1
Protein Refseq : NP_003001.1
UniProt ID : P45985
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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