Recombinant Full Length Human MDH1 Protein, C-Flag-tagged

• Cat.No.: MDH1-1976HFL
• Product Overview: Recombinant Full Length Human MDH1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 36.2 kDa
• AA Sequence: MSEPIRVLVTGAAGQIAYSLLYSIG NGSVFGKDQPIILVLLDITPMMGVL DGVLMELQDCALPLLKDVIA TDKEDVAFKDLDVAILVGSMPRREG MERKDLLKANVKIFKSQGAALDKYA KKSVKVIVVGNPANTNCLTA SKSAPSIPKENFSCLTRLDHNRAKA QIALKLGVTANDVKNVIIWGNHSST QYPDVNHAKVKLQGKEVGVY EALKDDSWLKGEFVTTVQQRGAAVI KARKLSSAMSAAKAICDHVRDIWFG TPEGEFVSMGVISDGNSYGV PDDLLYSFPVVIKNKTWKFVEGLPI NDFSREKMDLTAKELTEEKESAFEF LSSA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism

Gene Information

• Gene Name: MDH1 malate dehydrogenase 1 [ Homo sapiens (human) ]
• Official Symbol: MDH1
• Synonyms: KAR; MDHA; MOR2; DEE88; MDH-s; EIEE88; HEL-S-32; MGC:1375
• Gene ID: 4190
• mRNA Refseq: NM_005917.4
• Protein Refseq: NP_005908.1
• MIM: 154200
• UniProt ID: P40925