Recombinant Full Length Human MITD1 Protein, C-Flag-tagged

• Cat.No.: MITD1-2159HFL
• Product Overview: Recombinant Full Length Human MITD1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Abscission, the separation of daughter cells at the end of cytokinesis, is effected by endosomal sorting complexes required for transport III (ESCRT-III). The protein encoded by this gene functions as a homodimer, with the N-termini binding to a subset of ESCRT-III subunits and the C-termini binding to membranes. The encoded protein regulates ESCRT-III activity and is required for proper cytokinesis. Several transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 29.1 kDa
• AA Sequence: MAKSGLRQDPQSTAAATVLKRAVEL DSESRYPQALVCYQEGIDLLLQVLK GTKDNTKRCNLREKISKYMD RAENIKKYLDQEKEDGKYHKQIKIE ENATGFSYESLFREYLNETVTEVWI EDPYIRHTHQLYNFLRFCEM LIKRPCKVKTIHLLTSLDEGIEQVQ QSRGLQEIEESLRSHGVLLEVQYSS SIHDREIRFNNGWMIKIGRG LDYFKKPQSRFSLGYCDFDLRPCHE TTVDIFHKKHTKNI TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: MITD1 microtubule interacting and trafficking domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: MITD1
• Synonyms: domain containing 1; microtubule interacting and transport; MIT
• Gene ID: 129531
• mRNA Refseq: NM_138798.3
• Protein Refseq: NP_620153.1
• UniProt ID: Q8WV92