| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Flag |
| Description : |
Enables magnesium ion transmembrane transporter activity. Involved in mitochondrial magnesium ion transmembrane transport. Located in mitochondrion. |
| Molecular Mass : |
50.1 kDa |
| Storage : |
The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20 centigrade. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. |
| Cytogenetics : |
6p22.3 |
| Preparation : |
HEK293T cells in 10-cm dishes were transiently transfected with 5ug cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Protein Families : |
Transcription Factors, Transmembrane |
| Components : |
1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate. |
