Recombinant Full Length Human MTHFD1 Protein, C-Flag-tagged

• Cat.No.: MTHFD1-282HFL
• Product Overview: Recombinant Full Length Human MTHFD1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 101.4 kDa
• AA Sequence: MAPAEILNGKEISAQIRARLKNQVT QLKEQVPGFTPRLAILQVGNRDDSN LYINVKLKAAEEIGIKATHI KLPRTTTESEVMKYITSLNEDSTVH GFLVQLPLDSENSINTEEVINAIAP EKDVDGLTSINAGRLARGDL NDCFIPCTPKGCLELIKETGVPIAG RHAVVVGRSKIVGAPMHDLLLWNNA TVTTCHSKTAHLDEEVNKGD ILVVATGQPEMVKGEWIKPGAIVID CGINYVPDDKKPNGRKVVGDVAYDE AKERASFITPVPGGVGPMTV AMLMQSTVESAKRFLEKFKPGKWMI QYNNLNLKTPVPSDIDISRSCKPKP IGKLAREIGLLSEEVELYGE TKAKVLLSALERLKHRPDGKYVVVT GITPTPLGEGKSTTTIGLVQALGAH LYQNVFACVRQPSQGPTFGI KGGAAGGGYSQVIPMEEFNLHLTGD IHAITAANNLVAAAIDARIFHELTQ TDKALFNRLVPSVNGVRRFS DIQIRRLKRLGIEKTDPTTLTDEEI NRFARLDIDPETITWQRVLDTNDRF LRKITIGQAPTEKGHTRTAQ FDISVASEIMAVLALTTSLEDMRER LGKMVVASSKKGEPVSAEDLGVSGA LTVLMKDAIKPNLMQTLEGT PVFVHAGPFANIAHGNSSIIADQIA LKLVGPEGFVVTEAGFGADIGMEKF FNIKCRYSGLCPHVVVLVAT VRALKMHGGGPTVTAGLPLPKAYIQ ENLELVEKGFSNLKKQIENARMFGI PVVVAVNAFKTDTESELDLI SRLSREHGAFDAVKCTHWAEGGKGA LALAQAVQRAAQAPSSFQLLYDLKL PVEDKIRIIAQKIYGADDIE LLPEAQHKAEVYTKQGFGNLPICMA KTHLSLSHNPEQKGVPTGFILPIRD IRASVGAGFLYPLVGTMSTM
  PGLPTRPCFYDIDLDPETEQVNGLF TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate

Gene Information

• Gene Name: MTHFD1 methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 [ Homo sapiens (human) ]
• Official Symbol: MTHFD1
• Synonyms: CIMAH; MTHFC; MTHFD
• Gene ID: 4522
• mRNA Refseq: NM_005956.4
• Protein Refseq: NP_005947.3
• MIM: 172460
• UniProt ID: P11586