Recombinant Full Length Human MYH3 Protein, C-Flag-tagged

• Cat.No.: MYH3-905HFL
• Product Overview: Recombinant Full Length Human MYH3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 223.7 kDa
• AA Sequence: MSSDTEMEVFGIAAPFLRKSEKERI EAQNQPFDAKTYCFVVDSKEEYAKG KIKSSQDGKVTVETEDNRTL VVKPEDVYAMNPPKFDRIEDMAMLT HLNEPAVLYNLKDRYTSWMIYTYSG LFCVTVNPYKWLPVYNPEVV EGYRGKKRQEAPPHIFSISDNAYQF MLTDRENQSILITGESGAGKTVNTK RVIQYFATIAATGDLAKKKD SKMKGTLEDQIISANPLLEAFGNAK TVRNDNSSRFGKFIRIHFGTTGKLA SADIETYLLEKSRVTFQLKA ERSYHIFYQILSNKKPELIELLLIT TNPYDYPFISQGEILVASIDDAEEL LATDSAIDILGFTPEEKSGL YKLTGAVMHYGNMKFKQKQREEQAE PDGTEVADKTAYLMGLNSSDLLKAL CFPRVKVGNEYVTKGQTVDQ VHHAVNALSKSVYEKLFLWMVTRIN QQLDTKLPRQHFIGVLDIAGFEIFE YNSLEQLCINFTNEKLQQFF NHHMFVLEQEEYKKEGIEWTFIDFG MDLAACIELIEKPMGIFSILEEECM FPKATDTSFKNKLYDQHLGK SNNFQKPKVVKGRAEAHFSLIHYAG TVDYSVSGWLEKNKDPLNETVVGLY QKSSNRLLAHLYATFATADA DSGKKKVAKKKGSSFQTVSALFREN LNKLMSNLRTTHPHFVRCIIPNETK TPGAMEHSLVLHQLRCNGVL EGIRICRKGFPNRILYGDFKQRYRV LNASAIPEGQFIDSKKACEKLLASI DIDHTQYKFGHTKVFFKAGL LGTLEEMRDDRLAKLITRTQAVCRG FLMRVEFQKMVQRRESIFCIQYNIR SFMNVKHWPWMKLFFKIKPL LKSAETEKEMATMKEEFQKTKDELA KSEAKRKELEEKLVTLVQEKNDLQL QVQAESENLLDAEERCDQLI KAKFQLEAKIKEVTERAEDEEEINA ELTAKKRKLEDECSELKKDIDDLEL TLAKVEKEKHATENKVKNLT EELSGLDETIAKLTREKKALQEAHQ QALDDLQAEEDKVNSLNKTKSKLEQ QVEDLESSLEQEKKLRVDLE RNKRKLEGDLKLAQESILDLENDKQ QLDERLKKKDFEYCQLQSKVEDEQT LGLQFQKKIKELQARIEELE EEIEAERATRAKTEKQRSDYARELE ELSERLEEAGGVTSTQIELNKKREA EFLKLRRDLEEATLQHEAMV AALRKKHADSVAELGEQIDNLQRVK QKLEKEKSEFKLEIDDLSSSMESVS KSKANLEKICRTLEDQLSEA RGKNEEIQRSLSELTTQKSRLQTEA GELSRQLEEKESIVSQLSRSKQAFT QQTEELKRQLEEENKAKNAL AHALQSSRHDCDLLREQYEEEQEGK AELQRALSKANSEVAQWRTKYETDA IQRTEELEEAKKKLAQRLQD SEEQVEAVNAKCASLEKTKQRLQGE VEDLMVDVERANSLAAALDKKQRNF DKVLAEWKTKCEESQAELEA SLKESRSLSTELFKLKNAYEEALDQ LETVKRENKNLEQEIADLTEQIAEN GKTIHELEKSRKQIELEKAD IQLALEEAEAALEHEEAKILRIQLE LTQVKSEIDRKIAEKDEEIEQLKRN YQRTVETMQSALDAEVRSRN EAIRLKKKMEGDLNEIEIQLSHANR QAAETLKHLRSVQGQLKDTQLHLDD ALRGQEDLKEQLAIVERRAN LLQAEVEELRATLEQTERARKLAEQ ELLDSNERVQLLHTQNTSLIHTKKK LETDLMQLQSEVEDASRDAR NAEEKAKKAITDAAMMAEELKKEQD TSAHLERMKKNLEQTVKDLQHRLDE AEQLALKGGKKQIQKLETRI RELEFELEGEQKKNTESVKGLRKYE RRVKELTYQSEEDRKNVLRLQDLVD KLQVKVKSYKRQAEEADEQA
  NAHLTKFRKAQHELEEAEERADIAE SQVNKLRAKTRDFTSSRMVVHESEE TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Tight junction, Viral myocarditis

Gene Information

• Gene Name: MYH3 myosin heavy chain 3 [ Homo sapiens (human) ]
• Official Symbol: MYH3
• Synonyms: DA8; DA2A; DA2B; DA2B3; HEMHC; SMHCE; MYHSE1; CPSFS1A; CPSFS1B; CPSKF1A; CPSKF1B; MYHC-EMB
• Gene ID: 4621
• mRNA Refseq: NM_002470.4
• Protein Refseq: NP_002461.2
• MIM: 160720
• UniProt ID: P11055