Recombinant Human MYH3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MYH3-1840H
• Product Overview: MYH3 MS Standard C13 and N15-labeled recombinant protein (NP_002461) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 224.4 kDa
• AA Sequence: MSSDTEMEVFGIAAPFLRKSEKERI EAQNQPFDAKTYCFVVDSKEEYAKG KIKSSQDGKVTVETEDNRTLVVKPE DVYAMNPPKFDRIEDMAMLTHLNEP AVLYNLKDRYTSWMIYTYSGLFCVT VNPYKWLPVYNPEVVEGYRGKKRQE APPHIFSISDNAYQFMLTDRENQSI LITGESGAGKTVNTKRVIQYFATIA ATGDLAKKKDSKMKGTLEDQIISAN PLLEAFGNAKTVRNDNSSRFGKFIR IHFGTTGKLASADIETYLLEKSRVT FQLKAERSYHIFYQILSNKKPELIE LLLITTNPYDYPFISQGEILVASID DAEELLATDSAIDILGFTPEEKSGL YKLTGAVMHYGNMKFKQKQREEQAE PDGTEVADKTAYLMGLNSSDLLKAL CFPRVKVGNEYVTKGQTVDQVHHAV NALSKSVYEKLFLWMVTRINQQLDT KLPRQHFIGVLDIAGFEIFEYNSLE QLCINFTNEKLQQFFNHHMFVLEQE EYKKEGIEWTFIDFGMDLAACIELI EKPMGIFSILEEECMFPKATDTSFK NKLYDQHLGKSNNFQKPKVVKGRAE AHFSLIHYAGTVDYSVSGWLEKNKD PLNETVVGLYQKSSNRLLAHLYATF ATADADSGKKKVAKKKGSSFQTVSA LFRENLNKLMSNLRTTHPHFVRCII PNETKTPGAMEHSLVLHQLRCNGVL EGIRICRKGFPNRILYGDFKQRYRV LNASAIPEGQFIDSKKACEKLLASI DIDHTQYKFGHTKVFFKAGLLGTLE EMRDDRLAKLITRTQAVCRGFLMRV EFQKMVQRRESIFCIQYNIRSFMNV KHWPWMKLFFKIKPLLKSAETEKEM ATMKEEFQKTKDELAKSEAKRKELE EKLVTLVQEKNDLQLQVQAESENLL DAEERCDQLIKAKFQLEAKIKEVTE RAEDEEEINAELTAKKRKLEDECSE LKKDIDDLELTLAKVEKEKHATENK VKNLTEELSGLDETIAKLTREKKAL QEAHQQALDDLQAEEDKVNSLNKTK SKLEQQVEDLESSLEQEKKLRVDLE RNKRKLEGDLKLAQESILDLENDKQ QLDERLKKKDFEYCQLQSKVEDEQT LGLQFQKKIKELQARIEELEEEIEA ERATRAKTEKQRSDYARELEELSER LEEAGGVTSTQIELNKKREAEFLKL RRDLEEATLQHEAMVAALRKKHADS VAELGEQIDNLQRVKQKLEKEKSEF KLEIDDLSSSMESVSKSKANLEKIC RTLEDQLSEARGKNEEIQRSLSELT TQKSRLQTEAGELSRQLEEKESIVS QLSRSKQAFTQQTEELKRQLEEENK AKNALAHALQSSRHDCDLLREQYEE EQEGKAELQRALSKANSEVAQWRTK YETDAIQRTEELEEAKKKLAQRLQD SEEQVEAVNAKCASLEKTKQRLQGE VEDLMVDVERANSLAAALDKKQRNF DKVLAEWKTKCEESQAELEASLKES RSLSTELFKLKNAYEEALDQLETVK RENKNLEQEIADLTEQIAENGKTIH ELEKSRKQIELEKADIQLALEEAEA ALEHEEAKILRIQLELTQVKSEIDR KIAEKDEEIEQLKRNYQRTVETMQS ALDAEVRSRNEAIRLKKKMEGDLNE IEIQLSHANRQAAETLKHLRSVQGQ LKDTQLHLDDALRGQEDLKEQLAIV ERRANLLQAEVEELRATLEQTERAR KLAEQELLDSNERVQLLHTQNTSLI HTKKKLETDLMQLQSEVEDASRDAR NAEEKAKKAITDAAMMAEELKKEQD TSAHLERMKKNLEQTVKDLQHRLDE AEQLALKGGKKQIQKLETRIRELEF ELEGEQKKNTESVKGLRKYERRVKE LTYQSEEDRKNVLRLQDLVDKLQVK VKSYKRQAEEADEQANAHLTKFRKA QHELEEAEERADIAESQVNKLRAKT RDFTSSRMVVHESEETRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MYH3 myosin heavy chain 3 [ Homo sapiens (human) ]
• Official Symbol: MYH3
• Synonyms: MYH3; myosin, heavy chain 3, skeletal muscle, embryonic; myosin-3; myosin, skeletal, heavy chain, embryonic 1; myosin heavy chain, fast skeletal muscle, embryonic; myosin, heavy polypeptide 3, skeletal muscle, embryonic; HEMHC; SMHCE; MYHSE1; MYHC-EMB
• Gene ID: 4621
• mRNA Refseq: NM_002470
• Protein Refseq: NP_002461
• MIM: 160720
• UniProt ID: P11055