Recombinant Full Length Human NAGS Protein, C-Flag-tagged

• Cat.No.: NAGS-1012HFL
• Product Overview: Recombinant Full Length Human NAGS Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 58 kDa
• AA Sequence: MATALMAVVLRAAAVAPRLRGRGGT GGARRLSCGARRRAARGTSPGRRLS TAWSQPQPPPEEYAGADDVS QSPVAEEPSWVPSPRPPVPHESPEP PSGRSLVQRDIQAFLNQCGASPGEA RHWLTQFQTCHHSADKPFAV IEVDEEVLKCQQGVSSLAFALAFLQ RMDMKPLVVLGLPAPTAPSGCLSFW EAKAQLAKSCKVLVDALRHN AAAAVPFFGGGSVLRAAEPAPHASY GGIVSVETDLLQWCLESGSIPILCP IGETAARRSVLLDSLEVTAS LAKALRPTKIIFLNNTGGLRDSSHK VLSNVNLPADLDLVCNAEWVSTKER QQMRLIVDVLSRLPHHSSAV ITAASTLLTELFSNKGSGTLFKNAE RMLRVRSLDKLDQGRLVDLVNASFG KKLRDDYLASLRPRLHSIYV SEGYNAAAILTMEPVLGGTPYLDKF VVSSSRQGQGSGQMLWECLRRDLQT LFWRSRVTNPINPWYFKHSD
  GSFSNKQWIFFWFGLADIRDSYELV NHAKGLPDSFHKPASDPGSTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways

Gene Information

• Gene Name: NAGS N-acetylglutamate synthase [ Homo sapiens (human) ]
• Official Symbol: NAGS
• Synonyms: AGAS; ARGA
• Gene ID: 162417
• mRNA Refseq: NM_153006.3
• Protein Refseq: NP_694551.1
• MIM: 608300
• UniProt ID: Q8N159