Recombinant Full Length Human NCSTN Protein, C-Flag-tagged

• Cat.No.: NCSTN-1840HFL
• Product Overview: Recombinant Full Length Human NCSTN Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 75.2 kDa
• AA Sequence: MATAGGGSGADPGSRGLLRLLSFCV LLAGLCRGNSVERKIYIPLNKTAPC VRLLNATHQIGCQSSISGDT GVIHVVEKEEDLQWVLTDGPNPPYM VLLESKHFTRDLMEKLKGRTSRIAG LAVSLTKPSPASGFSPSVQC PNDGFGVYSNSYGPEFAHCREIQWN SLGNGLAYEDFSFPIFLLEDENETK VIKQCYQDHNLSQNGSAPTF PLCAMQLFSHMHAVISTATCMRRSS IQSTFSINPEIVCDPLSDYNVWSML KPINTTGTLKPDDRVVVAAT RLDSRSFFWNVAPGAESAVASFVTQ LAAAEALQKAPDVTTLPRNVMFVFF QGETFDYIGSSRMVYDMEKG KFPVQLENVDSFVELGQVALRTSLE LWMHTDPVSQKNESVRNQVEDLLAT LEKSGAGVPAVILRRPNQSQ PLPPSSLQRFLRARNISGVVLADHS GAFHNKYYQSIYDTAENINVSYPEW LSPEEDLNFVTDTAKALADV ATVLGRALYELAGGTNFSDTVQADP QTVTRLLYGFLIKANNSWFQSILRQ DLRSYLGDGPLQHYIAVSSP TNTTYVVQYALANLTGTVVNLTREQ CQDPSKVPSENKDLYEYSWVQGPLH SNETDRLPRCVRSTARLARA LSPAFELSQWSSTEYSTWTESRWKD IRARIFLIASKELELITLTVGFGIL IFSLIVTYCINAKADVLFIA PREPGAVSY TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Notch signaling pathway

Gene Information

• Gene Name: NCSTN nicastrin [ Homo sapiens (human) ]
• Official Symbol: NCSTN
• Synonyms: ATAG1874
• Gene ID: 23385
• mRNA Refseq: NM_015331.3
• Protein Refseq: NP_056146.1
• MIM: 605254
• UniProt ID: Q92542