Recombinant Full Length Human NLRP10 Protein, C-Flag-tagged

• Cat.No.: NLRP10-1119HFL
• Product Overview: Recombinant Full Length Human NLRP10 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). The protein encoded by this gene belongs to the NALP protein family despite lacking the LRR region. This protein likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. Other experiments indicate that this gene acts as a multifunctional negative regulator of inflammation and apoptosis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 74.9 kDa
• AA Sequence: MAMAKARKPREALLWALSDLEENDF KKLKFYLRDMTLSEGQPPLARGELE GLIPVDLAELLISKYGEKEA VKVVLKGLKVMNLLELVDQLSHICL HDYREVYREHVRCLEEWQEAGVNGR YNQVLLVAKPSSESPESLAC PFPEQELESVTVEALFDSGEKPSLA PSLVVLQGSAGTGKTTLARKMVLDW ATGTLYPGRFDYVFYVSCKE VVLLLESKLEQLLFWCCGDNQAPVT EILRQPERLLFILDGFDELQRPFEE KLKKRGLSPKESLLHLLIRR HTLPTCSLLITTRPLALRNLEPLLK QARHVHILGFSEEERARYFSSYFTD EKQADRAFDIVQKNDILYKA CQVPGICWVVCSWLQGQMERGKVVL ETPRNSTDIFMAYVSTFLPPDDDGG CSELSRHRVLRSLCSLAAEG IQHQRFLFEEAELRKHNLDGPRLAA FLSSNDYQLGLAIKKFYSFRHISFQ DFFHAMSYLVKEDQSRLGKE SRREVQRLLEVKEQEGNDEMTLTMQ FLLDISKKDSFSNLELKFCFRISPC LAQDLKHFKEQMESMKHNRT WDLEFSLYEAKIKNLVKGIQMNNVS FKIKHSNEKKSQSQNLFSVKSSLSH GPKEEQKCPSVHGQKEGKDN
  IAGTQKEASTGKGRGTEETPKNTYI TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: NLRP10 NLR family pyrin domain containing 10 [ Homo sapiens (human) ]
• Official Symbol: NLRP10
• Synonyms: NOD8; PAN5; PYNOD; NALP10; CLR11.1
• Gene ID: 338322
• mRNA Refseq: NM_176821.4
• Protein Refseq: NP_789791.1
• MIM: 609662
• UniProt ID: Q86W26