Recombinant Full Length Human NR1I2 Protein, C-Flag-tagged

• Cat.No.: NR1I2-781HFL
• Product Overview: Recombinant Full Length Human NR1I2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 49.6 kDa
• AA Sequence: AIAXEVRPKESWNHADFVHCEDTES VPGKPSVNADEEVGGPQICRVCGDK ATGYHFNVMTCEGCKGFFRR AMKRNARLRCPFRKGACEITRKTRR QCQACRLRKCLESGMKKEMIMSDEA VEERRALIKRKKSERTGTQP LGVQGLTEEQRMMIRELMDAQMKTF DTTFSHFKNFRPGVLSSGCELPESL QAPSREEAAKWSQVRKDLCS LKVSLQLRGEDGSVWNYKPPADSGG KEIFSLLPHMADMSTYMFKGIISFA KVISYFRDLPIEDQISLLKG AAFELCQLRFNTVFNAETGTWECGR LSYCLEDTAGGFQQLLLEPMLKFHY MLKKLQLHEEEYVLMQAISL FSPDRPGVLQHRVVDQLQEQFAITL KSYIECNRPQPAHRFLFLKIMAMLT ELRSINAQHTQRLLRIQDIH
  PFATPLMQELFGITGSTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

Gene Information

• Gene Name: NR1I2 nuclear receptor subfamily 1 group I member 2 [ Homo sapiens (human) ]
• Official Symbol: NR1I2
• Synonyms: BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq
• Gene ID: 8856
• mRNA Refseq: NM_003889.4
• Protein Refseq: NP_003880.3
• MIM: 603065
• UniProt ID: O75469