Recombinant Full Length Human NUDT15 Protein, C-Flag-tagged

• Cat.No.: NUDT15-892HFL
• Product Overview: Recombinant Full Length Human NUDT15 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 18.4 kDa
• AA Sequence: MTASAQPRGRRPGVGVGVVVTSCKH PRCVLLGKRKGSVGAGSFQLPGGHL EFGETWEECAQRETWEEAAL HLKNVHFASVVNSFIEKENYHYVTI LMKGEVDVTHDSEPKNVEPEKNESW EWVPWEELPPLDQLFWGLRC
  LKEQGYDPFKEDLNHLVGYKGNHLT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: NUDT15 nudix hydrolase 15 [ Homo sapiens (human) ]
• Official Symbol: NUDT15
• Synonyms: MTH2; NUDT15D
• Gene ID: 55270
• mRNA Refseq: NM_018283.4
• Protein Refseq: NP_060753.1
• MIM: 615792
• UniProt ID: Q9NV35