Recombinant Full Length Human PARN Protein, C-Flag-tagged

• Cat.No.: PARN-548HFL
• Product Overview: Recombinant Full Length Human PARN Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 73.3 kDa
• AA Sequence: MEIIRSNFKSNLHKVYQAIEEADFF AIDGEFSGISDGPSVSALTNGFDTP EERYQKLKKHSMDFLLFQFG LCTFKYDYTDSKYITKSFNFYVFPK PFNRSSPDVKFVCQSSSIDFLASQG FDFNKVFRNGIPYLNQEEER QLREQYDEKRSQANGAGALSYVSPN TSKCPVTIPEDQKKFIDQVVEKIED LLQSEENKNLDLEPCTGFQR KLIYQTLSWKYPKGIHVETLETEKK ERYIVISKVDEEERKRREQQKHAKE QEELNDAVGFSRVIHAIANS GKLVIGHNMLLDVMHTVHQFYCPLP ADLSEFKEMTTCVFPRLLDTKLMAS TQPFKDIINNTSLAELEKRL KETPFNPPKVESAEGFPSYDTASEQ LHEAGYDAYITGLCFISMANYLGSF LSPPKIHVSARSKLIEPFFN KLFLMRVMDIPYLNLEGPDLQPKRD HVLHVTFPKEWKTSDLYQLFSAFGN IQISWIDDTSAFVSLSQPEQ VKIAVNTSKYAESYRIQTYAEYMGR KQEEKQIKRKWTEDSWKEADSKRLN PQCIPYTLQNHYYRNNSFTA PSTVGKRNLSPSQEEAGLEDGVSGE ISDTELEQTDSCAEPLSEGRKKAKK LKRMKKELSPAGSISKNSPA
  TLFEVPDTWTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation

Gene Information

• Gene Name: PARN poly(A)-specific ribonuclease [ Homo sapiens (human) ]
• Official Symbol: PARN
• Synonyms: DAN; DKCB6; PFBMFT4
• Gene ID: 5073
• mRNA Refseq: NM_002582.4
• Protein Refseq: NP_002573.1
• MIM: 604212
• UniProt ID: O95453