Recombinant Full Length Human PDIA3 Protein, C-Flag-tagged

• Cat.No.: PDIA3-871HFL
• Product Overview: Recombinant Full Length Human PDIA3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.2 kDa
• AA Sequence: MRLRRLALFPGVALLLAAARLAAAS DVLELTDDNFESRISDTGSAGLMLV EFFAPWCGHCKRLAPEYEAA ATRLKGIVPLAKVDCTANTNTCNKY GVSGYPTLKIFRDGEEAGAYDGPRT ADGIVSHLKKQAGPASVPLR TEEEFKKFISDKDASIVGFFDDSFS EAHSEFLKAASNLRDNYRFAHTNVE SLVNEYDDNGEGIILFRPSH LTNKFEDKTVAYTEQKMTSGKIKKF IQENIFGICPHMTEDNKDLIQGKDL LIAYYDVDYEKNAKGSNYWR NRVMMVAKKFLDAGHKLNFAVASRK TFSHELSDFGLESTAGEIPVVAIRT AKGEKFVMQEEFSRDGKALE RFLQDYFDGNLKRYLKSEPIPESND GPVKVVVAENFDEIVNNENKDVLIE FYAPWCGHCKNLEPKYKELG EKLSKDPNIVIAKMDATANDVPSPY EVRGFPTIYFSPANKKLNPKKYEGG RELSDFISYLQREATNPPVI
  QEEKPKKKKKAQEDLTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation

Gene Information

• Gene Name: PDIA3 protein disulfide isomerase family A member 3 [ Homo sapiens (human) ]
• Official Symbol: PDIA3
• Synonyms: P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• Gene ID: 2923
• mRNA Refseq: NM_005313.5
• Protein Refseq: NP_005304.3
• MIM: 602046
• UniProt ID: P30101