Recombinant Full Length Human PGK1 Protein, C-Flag-tagged

• Cat.No.: PGK1-1824HFL
• Product Overview: Recombinant Full Length Human PGK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 44.4 kDa
• AA Sequence: MSLSNKLTLDKLDVKGKRVVMRVDF NVPMKNNQITNNQRIKAAVPSIKFC LDNGAKSVVLMSHLGRPDGV PMPDKYSLEPVAVELKSLLGKDVLF LKDCVGPEVEKACANPAAGSVILLE NLRFHVEEEGKGKDASGNKV KAEPAKIEAFRASLSKLGDVYVNDA FGTAHRAHSSMVGVNLPQKAGGFLM KKELNYFAKALESPERPFLA ILGGAKVADKIQLINNMLDKVNEMI IGGGMAFTFLKVLNNMEIGTSLFDE EGAKIVKDLMSKAEKNGVKI TLPVDFVTADKFDENAKTGQATVAS GIPAGWMGLDCGPESSKKYAEAVTR AKQIVWNGPVGVFEWEAFAR GTKALMDEVVKATSRGCITIIGGGD TATCCAKWNTEDKVSHVSTGGGASL ELLEGKVLPGVDALSNI TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways

Gene Information

• Gene Name: PGK1 phosphoglycerate kinase 1 [ Homo sapiens (human) ]
• Official Symbol: PGK1
• Synonyms: PGKA; MIG10; HEL-S-68p
• Gene ID: 5230
• mRNA Refseq: NM_000291.4
• Protein Refseq: NP_000282.1
• MIM: 311800
• UniProt ID: P00558