Recombinant Full Length Human PLOD1 Protein, C-Flag-tagged

• Cat.No.: PLOD1-843HFL
• Product Overview: Recombinant Full Length Human PLOD1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 81.5 kDa
• AA Sequence: MRPLLLLALLGWLLLAEAKGDAKPE DNLLVLTVATKETEGFRRFKRSAQF FNYKIQALGLGEDWNVEKGT SAGGGQKVRLLKKALEKHADKEDLV ILFTDSYDVLFASGPRELLKKFRQS RSQVVFSAEELIYPDRRLET KYPVVSDGKRFLGSGGFIGYAPNLS KLVAEWEGQDSDSDQLFYTKIFLDP EKREQINITLDHRCRIFQNL DGALDEVVLKFEMGHVRARNLAYDT LPVLIHGNGPTKLQLNYLGNYIPRF WTFETGCTVCDEGLRSLKGI GDEALPTVLVGVFIEQPTPFVSLFF QRLLRLHYPQKHMRLFIHNHEQHHK AQVEEFLAQHGSEYQSVKLV GPEVRMANADARNMGADLCRQDRSC TYYFSVDADVALTEPNSLRLLIQQN KNVIAPLMTRHGRLWSNFWG ALSADGYYARSEDYVDIVQGRRVGV WNVPYISNIYLIKGSALRGELQSSD LFHHSKLDPDMAFCANIRQQ DVFMFLTNRHTLGHLLSLDSYRTTH LHNDLWEVFSNPEDWKEKYIHQNYT KALAGKLVETPCPDVYWFPI FTEVACDELVEEMEHFGQWSLGNNK DNRIQGGYENVPTIDIHMNQIGFER EWHKFLLEYIAPMTEKLYPG YYTRAQFDLAFVVRYKPDEQPSLMP HHDASTFTINIALNRVGVDYEGGGC RFLRYNCSIRAPRKGWTLMH
  PGRLTHYHEGLPTTRGTRYIAVSFV DPTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation

Gene Information

• Gene Name: PLOD1 procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 [ Homo sapiens (human) ]
• Official Symbol: PLOD1
• Synonyms: LH; LH1; LLH; EDS6; PLOD; EDSKCL1
• Gene ID: 5351
• mRNA Refseq: NM_000302.4
• Protein Refseq: NP_000293.2
• MIM: 153454
• UniProt ID: Q02809