Recombinant Full Length Human PNMT Protein, C-Flag-tagged

• Cat.No.: PNMT-1440HFL
• Product Overview: Recombinant Full Length Human PNMT Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 30.7 kDa
• AA Sequence: MSGADRSPNAGAAPDSAPGQAAVAS AYQRFEPRAYLRNNYAPPRGDLCNP NGVGPWKLRCLAQTFATGEV SGRTLIDIGSGPTVYQLLSACSHFE DITMTDFLEVNRQELGRWLQEEPGA FNWSMYSQHACLIEGKGECW QDKERQLRARVKRVLPIDVHQPQPL GAGSPAPLPADALVSAFCLEAVSPD LASFQRALDHITTLLRPGGH LLLIGALEESWYLAGEARLTVVPVS EEEVREALVRSGYKVRDLRTYIMPA HLQTGVDDVKGVFFAWAQKV
  GLTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism

Gene Information

• Gene Name: PNMT phenylethanolamine N-methyltransferase [ Homo sapiens (human) ]
• Official Symbol: PNMT
• Synonyms: PENT; PNMTase
• Gene ID: 5409
• mRNA Refseq: NM_002686.4
• Protein Refseq: NP_002677.1
• MIM: 171190
• UniProt ID: P11086