Recombinant Full Length Human PPP2R1A Protein, C-Flag-tagged

• Cat.No.: PPP2R1A-281HFL
• Product Overview: Recombinant Full Length Human PPP2R1A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 65.1 kDa
• AA Sequence: MAAADGDDSLYPIAVLIDELRNEDV QLRLNSIKKLSTIALALGVERTRSE LLPFLTDTIYDEDEVLLALA EQLGTFTTLVGGPEYVHCLLPPLES LATVEETVVRDKAVESLRAISHEHS PSDLEAHFVPLVKRLAGGDW FTSRTSACGLFSVCYPRVSSAVKAE LRQYFRNLCSDDTPMVRRAAASKLG EFAKVLELDNVKSEIIPMFS NLASDEQDSVRLLAVEACVNIAQLL PQEDLEALVMPTLRQAAEDKSWRVR YMVADKFTELQKAVGPEITK TDLVPAFQNLMKDCEAEVRAAASHK VKEFCENLSADCRENVIMSQILPCI KELVSDANQHVKSALASVIM GLSPILGKDNTIEHLLPLFLAQLKD ECPEVRLNIISNLDCVNEVIGIRQL SQSLLPAIVELAEDAKWRVR LAIIEYMPLLAGQLGVEFFDEKLNS LCMAWLVDHVYAIREAATSNLKKLV EKFGKEWAHATIIPKVLAMS GDPNYLHRMTTLFCINVLSEVCGQD ITTKHMLPTVLRMAGDPVANVRFNV AKSLQKIGPILDNSTLQSEV
  KPILEKLTQDQDVDVKYFAQEALTV LSLATRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway

Gene Information

• Gene Name: PPP2R1A protein phosphatase 2 scaffold subunit Aalpha [ Homo sapiens (human) ]
• Official Symbol: PPP2R1A
• Synonyms: MRD36; PP2AA; PR65A; PP2AAALPHA; PP2A-Aalpha
• Gene ID: 5518
• mRNA Refseq: NM_014225.6
• Protein Refseq: NP_055040.2
• MIM: 605983
• UniProt ID: P30153