Recombinant Full Length Human PREX2 Protein, C-Flag-tagged

• Cat.No.: PREX2-1930HFL
• Product Overview: Recombinant Full Length Human PREX2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 182.4 kDa
• AA Sequence: MSEDSRGDSRAESAKDLEKQLRLRV CVLSELQKTERDYVGTLEFLVSAFL HRMNQCAASKVDKNVTEETV KMLFSNIEDILAVHKEFLKVVEECL HPEPNAQQEVGTCFLHFKDKFRIYD EYCSNHEKAQKLLLELNKIR TIRTFLLNCMLLGGRKNTDVPLEGY LVTPIQRICKYPLILKELLKRTPRK HSDYAAVMEALQAMKAVCSN INEAKRQMEKLEVLEEWQSHIEGWE GSNITDTCTEMLMCGVLLKISSGNI QERVFFLFDNLLVYCKRKHR RLKNSKASTDGHRYLFRGRINTEVM EVENVDDGTADFHSSGHIVVNGWKI HNTAKNKWFVCMAKTPEEKH EWFEAILKERERRKGLKLGMEQDTW VMISEQGEKLYKMMCRQGNLIKDRK RKLTTFPKCFLGSEFVSWLL EIGEIHRPEEGVHLGQALLENGIIH HVTDKHQFKPEQMLYRFRYDDGTFY PRNEMQDVISKGVRLYCRLH SLFTPVIRDKDYHLRTYKSVVMANK LIDWLIAQGDCRTREEAMIFGVGLC DNGFMHHVLEKSEFKDEPLL FRFFSDEEMEGSNMKHRLMKHDLKV VENVIAKSLLIKSNEGSYGFGLEDK NKVPIIKLVEKGSNAEMAGM EVGKKIFAINGDLVFMRPFNEVDCF LKSCLNSRKPLRVLVSTKPRETVKI PDSADGLGFQIRGFGPSVVH AVGRGTVAAAAGLHPGQCIIKVNGI NVSKETHASVIAHVTACRKYRRPTK QDSIQWVYNSIESAQEDLQK SHSKPPGDEAGDAFDCKVEEVIDKF NTMAIIDGKKEHVSLTVDNVHLEYG VVYEYDSTAGIKCNVVEKMI EPKGFFSLTAKILEALAKSDEHFVQ NCTSLNSLNEVIPTDLQSKFSALCS ERIEHLCQRISSYKKFSRVL KNRAWPTFKQAKSKISPLHSSDFCP TNCHVNVMEVSYPKTSTSLGSAFGV QLDSRKHNSHDKENKSSEQG KLSPMVYIQHTITTMAAPSGLSLGQ QDGHGLRYLLKEEDLETQDIYQKLL GKLQTALKEVEMCVCQIDDL LSSITYSPKLERKTSEGIIPTDSDN EKGERNSKRVCFNVAGDEQEDSGHD TISNRDSYSDCNSNRNSIAS FTSICSSQCSSYFHSDEMDSGDELP LSVRISHDKQDKIHSCLEHLFSQVD SITNLLKGQAVVRAFDQTKY LTPGRGLQEFQQEMEPKLSCPKRLR LHIKQDPWNLPSSVRTLAQNIRKFV EEVKCRLLLALLEYSDSETQ LRRDMVFCQTLVATVCAFSEQLMAA LNQMFDNSKENEMETWEASRRWLDQ IANAGVLFHFQSLLSPNLTD EQAMLEDTLVALFDLEKVSFYFKPS EEEPLVANVPLTYQAEGSRQALKVY FYIDSYHFEQLPQRLKNGGG FKIHPVLFAQALESMEGYYYRDNVS VEEFQAQINAASLEKVKQYNQKLRA FYLDKSNSPPNSTSKAAYVD KLMRPLNALDELYRLVASFIRSKRT AACANTACSASGVGLLSVSSELCNR LGACHIIMCSSGVHRCTLSV TLEQAIILARSHGLPPRYIMQATDV MRKQGARVQNTAKNLGVRDRTPQSA PRLYKLCEPPPPAGEE TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PREX2 phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2 [ Homo sapiens (human) ]
• Official Symbol: PREX2
• Synonyms: DEP.2; DEPDC2; P-REX2; PPP1R129
• Gene ID: 80243
• mRNA Refseq: NM_024870.4
• Protein Refseq: NP_079146.2
• MIM: 612139
• UniProt ID: Q70Z35