| Description : |
The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
182.4 kDa |
| AA Sequence : |
MSEDSRGDSRAESAKDLEKQLRLRV CVLSELQKTERDYVGTLEFLVSAFL HRMNQCAASKVDKNVTEETVKMLFS NIEDILAVHKEFLKVVEECLHPEPN AQQEVGTCFLHFKDKFRIYDEYCSN HEKAQKLLLELNKIRTIRTFLLNCM LLGGRKNTDVPLEGYLVTPIQRICK YPLILKELLKRTPRKHSDYAAVMEA LQAMKAVCSNINEAKRQMEKLEVLE EWQSHIEGWEGSNITDTCTEMLMCG VLLKISSGNIQERVFFLFDNLLVYC KRKHRRLKNSKASTDGHRYLFRGRI NTEVMEVENVDDGTADFHSSGHIVV NGWKIHNTAKNKWFVCMAKTPEEKH EWFEAILKERERRKGLKLGMEQDTW VMISEQGEKLYKMMCRQGNLIKDRK RKLTTFPKCFLGSEFVSWLLEIGEI HRPEEGVHLGQALLENGIIHHVTDK HQFKPEQMLYRFRYDDGTFYPRNEM QDVISKGVRLYCRLHSLFTPVIRDK DYHLRTYKSVVMANKLIDWLIAQGD CRTREEAMIFGVGLCDNGFMHHVLE KSEFKDEPLLFRFFSDEEMEGSNMK HRLMKHDLKVVENVIAKSLLIKSNE GSYGFGLEDKNKVPIIKLVEKGSNA EMAGMEVGKKIFAINGDLVFMRPFN EVDCFLKSCLNSRKPLRVLVSTKPR ETVKIPDSADGLGFQIRGFGPSVVH AVGRGTVAAAAGLHPGQCIIKVNGI NVSKETHASVIAHVTACRKYRRPTK QDSIQWVYNSIESAQEDLQKSHSKP PGDEAGDAFDCKVEEVIDKFNTMAI IDGKKEHVSLTVDNVHLEYGVVYEY DSTAGIKCNVVEKMIEPKGFFSLTA KILEALAKSDEHFVQNCTSLNSLNE VIPTDLQSKFSALCSERIEHLCQRI SSYKKFSRVLKNRAWPTFKQAKSKI SPLHSSDFCPTNCHVNVMEVSYPKT STSLGSAFGVQLDSRKHNSHDKENK SSEQGKLSPMVYIQHTITTMAAPSG LSLGQQDGHGLRYLLKEEDLETQDI YQKLLGKLQTALKEVEMCVCQIDDL LSSITYSPKLERKTSEGIIPTDSDN EKGERNSKRVCFNVAGDEQEDSGHD TISNRDSYSDCNSNRNSIASFTSIC SSQCSSYFHSDEMDSGDELPLSVRI SHDKQDKIHSCLEHLFSQVDSITNL LKGQAVVRAFDQTKYLTPGRGLQEF QQEMEPKLSCPKRLRLHIKQDPWNL PSSVRTLAQNIRKFVEEVKCRLLLA LLEYSDSETQLRRDMVFCQTLVATV CAFSEQLMAALNQMFDNSKENEMET WEASRRWLDQIANAGVLFHFQSLLS PNLTDEQAMLEDTLVALFDLEKVSF YFKPSEEEPLVANVPLTYQAEGSRQ ALKVYFYIDSYHFEQLPQRLKNGGG FKIHPVLFAQALESMEGYYYRDNVS VEEFQAQINAASLEKVKQYNQKLRA FYLDKSNSPPNSTSKAAYVDKLMRP LNALDELYRLVASFIRSKRTAACAN TACSASGVGLLSVSSELCNRLGACH IIMCSSGVHRCTLSVTLEQAIILAR SHGLPPRYIMQATDVMRKQGARVQN TAKNLGVRDRTPQSAPRLYKLCEPP PPAGEETRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
