Recombinant Full Length Human PRKCZ Protein, C-Flag-tagged

• Cat.No.: PRKCZ-57HFL
• Product Overview: Recombinant Full Length Human PRKCZ Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 67.5 kDa
• AA Sequence: MPSRTGPKMEGSGGRVRLKAHYGGD IFITSVDAATTFEELCEEVRDMCRL HQQHPLTLKWVDSEGDPCTV SSQMELEEAFRLARQCRDEGLIIHV FPSTPEQPGLPCPGEDKSIYRRGAR RWRKLYRANGHLFQAKRFNR RAYCGQCSERIWGLARQGYRCINCK LLVHKRCHGLVPLTCRKHMDSVMPS QEPPVDDKNEDADLPSEETD GIAYISSSRKHDSIKDDSEDLKPVI DGMDGIKISQGLGLQDFDLIRVIGR GSYAKVLLVRLKKNDQIYAM KVVKKELVHDDEDIDWVQTEKHVFE QASSNPFLVGLHSCFQTTSRLFLVI EYVNGGDLMFHMQRQRKLPE EHARFYAAEICIALNFLHERGIIYR DLKLDNVLLDADGHIKLTDYGMCKE GLGPGDTTSTFCGTPNYIAP EILRGEEYGFSVDWWALGVLMFEMM AGRSPFDIITDNPDMNTEDYLFQVI LEKPIRIPRFLSVKASHVLK GFLNKDPKERLGCRPQTGFSDIKSH AFFRSIDWDLLEKKQALPPFQPQIT DDYGLDNFDTQFTSEPVQLT
  PDDEDAIKRIDQSEFEGFEYINPLL LSTEESVSGPTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis, Insulin signaling pathway, Tight junction, Type II diabetes mellitus

Gene Information

• Gene Name: PRKCZ protein kinase C zeta [ Homo sapiens (human) ]
• Official Symbol: PRKCZ
• Synonyms: PKC2; PKC-ZETA
• Gene ID: 5590
• mRNA Refseq: NM_002744.6
• Protein Refseq: NP_002735.3
• MIM: 176982
• UniProt ID: Q05513