Recombinant Full Length Human PSMF1 Protein, C-Flag-tagged

• Cat.No.: PSMF1-1546HFL
• Product Overview: Recombinant Full Length Human PSMF1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 29.6 kDa
• AA Sequence: MAGLEVLFASAAPAITCRQDALVCF LHWEVVTHGYFGLGVGDQPGPNDKK SELLPAGWNNNKDLYVLRYE YKDGSRKLLVKAITVESSMILNVLE YGSQQVADLTLNLDDYIDAEHLGDF HRTYKNSEELRSRIVSGIIT PIHEQWEKANVSSPHREFPPATARE VDPLRIPPRHPHTSRQPPWCDPLGP FVVGGEDLDPFGPRRGGMIV
  DPLRSGFPRALIDPSSGLPNRLPPG AVPPGARFDPFGPIGTSPPGPNPDH LPPPGYDDMYLTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Proteasome

Gene Information

• Gene Name: PSMF1 proteasome inhibitor subunit 1 [ Homo sapiens (human) ]
• Official Symbol: PSMF1
• Synonyms: PI31
• Gene ID: 9491
• mRNA Refseq: NM_006814.5
• Protein Refseq: NP_006805.2
• MIM: 617858
• UniProt ID: Q92530