Recombinant Full Length Human PTPRK Protein, C-Flag-tagged

• Cat.No.: PTPRK-1581HFL
• Product Overview: Recombinant Full Length Human PTPRK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP was shown to mediate homophilic intercellular interaction, possibly through the interaction with beta- and gamma-catenin at adherens junctions. Expression of this gene was found to be stimulated by TGF-beta 1, which may be important for the inhibition of keratinocyte proliferation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 160.3 kDa
• AA Sequence: MDTTAAAALPAFVALLLLSPWPLLG SAQGQFSAGGCTFDDGPGACDYHQD LYDDFEWVHVSAQEPHYLPP EMPQGSYMIVDSSDHDPGEKARLQL PTMKENDTHCIDFSYLLYSQKGLNP GTLNILVRVNKGPLANPIWN VTGFTGRDWLRAELAVSTFWPNEYQ VIFEAEVSGGRSGYIAIDDIQVLSY PCDKSPHFLRLGDVEVNAGQ NATFQCIATGRDAVHNKLWLQRRNG EDIPVAQTKNINHRRFAASFRLQEV TKTDQDLYRCVTQSERGSGV SNFAQLIVREPPRPIAPPQLLGVGP TYLLIQLNANSIIGDGPIILKEVEY RMTSGSWTETHAVNAPTYKL WHLDPDTEYEIRVLLTRPGEGGTGL PGPPLITRTKCAEPMRTPKTLKIAE IQARRIAVDWESLGYNITRC HTFNVTICYHYFRGHNESKADCLDM DPKAPQHVVNHLPPYTNVSLKMILT NPEGRKESEETIIQTDEDVP GPVPVKSLQGTSFENKIFLNWKEPL DPNGIITQYEISYSSIRSFDPAVPV AGPPQTVSNLWNSTHHVFMH LHPGTTYQFFIRASTVKGFGPATAI NVTTNISAPTLPDYEGVDASLNETA TTITVLLRPAQAKGAPISAY QIVVEELHPHRTKREAGAMECYQVP VTYQNAMSGGAPYYFAAELPPGNLP EPAPFTVGDNRTYQGFWNPP LAPRKGYNIYFQAMSSVEKETKTQC VRIATKAAATEEPEVIPDPAKQTDR VVKIAGISAGILVFILLLLV VILIVKKSKLAKKRKDAMGNTRQEM THMVNAMDRSYADQSTLHAEDPLSI TFMDQHNFSPRYENHSATAE SSRLLDVPRYLCEGTESPYQTGQLH PAIRVADLLQHINLMKTSDSYGFKE EYESFFEGQSASWDVAKKDQ NRAKNRYGNIIAYDHSRVILQPVED DPSSDYINANYIDIWLYRDGYQRPS HYIATQGPVHETVYDFWRMI WQEQSACIVMVTNLVEVGRVKCYKY WPDDTEVYGDFKVTCVEMEPLAEYV VRTFTLERRGYNEIREVKQF HFTGWPDHGVPYHATGLLSFIRRVK LSNPPSAGPIVVHCSAGAGRTGCYI VIDIMLDMAEREGVVDIYNC VKALRSRRINMVQTEEQYIFIHDAI LEACLCGETAIPVCEFKAAYFDMIR IDSQTNSSHLKDEFQTLNSV TPRLQAEDCSIACLPRNHDKNRFMD MLPPDRCLPFLITIDGESSNYINAA LMDSYRQPAAFIVTQYPLPN TVKDFWRLVYDYGCTSIVMLNEVDL SQGCPQYWPEEGMLRYGPIQVECMS CSMDCDVINRIFRICNLTRP QEGYLMVQQFQYLGWASHREVPGSK RSFLKLILQVEKWQEECEEGEGRTI IHCLNGGGRSGMFCAIGIVV
  EMVKRQNVVDVFHAVKTLRNSKPNM VEAPEQYRFCYDVALEYLESSSGPT RTRRLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase, Transmembrane

Gene Information

• Gene Name: PTPRK protein tyrosine phosphatase receptor type K [ Homo sapiens (human) ]
• Official Symbol: PTPRK
• Synonyms: R-PTP-kappa
• Gene ID: 5796
• mRNA Refseq: NM_001135648.3
• Protein Refseq: NP_001129120.1
• MIM: 602545
• UniProt ID: Q15262