Recombinant Full Length Human PTTG1 Protein, C-Flag-tagged

• Cat.No.: PTTG1-1819HFL
• Product Overview: Recombinant Full Length Human PTTG1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 21.8 kDa
• AA Sequence: MATLIYVDKENGEPGTRVVAKDGLK LGSGPSIKALDGRSQVSTPRFGKTF DAPPALPKATRKALGTVNRA TEKSVKTKGPLKQKQPSFSAKKMTE KTVKAKSSVPASDDAYPEIEKFFPF NPLDFESFDLPEEHQIAHLP LSGVPLMILDEERELEKLFQLGPPS PVKMPSPPWESNLLQSPSSILSTLD VELPPVCCDIDI TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, Oocyte meiosis

Gene Information

• Gene Name: PTTG1 PTTG1 regulator of sister chromatid separation, securin [ Homo sapiens (human) ]
• Official Symbol: PTTG1
• Synonyms: EAP1; PTTG; ECRAR; HPTTG; TUTR1
• Gene ID: 9232
• mRNA Refseq: NM_004219.4
• Protein Refseq: NP_004210.1
• MIM: 604147
• UniProt ID: O95997