Recombinant Full Length Human RAD54L Protein, C-Flag-tagged

• Cat.No.: RAD54L-1549HFL
• Product Overview: Recombinant Full Length Human RAD54L Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 84.2 kDa
• AA Sequence: MRRSLAPSQLAKRKPEGRSCDDEDW QPGLVTPRKRKSSSETQIQECFLSP FRKPLSQLTNQPPCLDSSQH EAFIRSILSKPFKVPIPNYQGPLGS RALGLKRAGVRRALHDPLEKDALVL YEPPPLSAHDQLKLDKEKLP VHVVVDPILSKVLRPHQREGVKFLW ECVTSRRIPGSHGCIMADEMGLGKT LQCITLMWTLLRQSPECKPE IDKAVVVSPSSLVKNWYNEVGKWLG GRIQPLAIDGGSKDEIDQKLEGFMN QRGARVSSPILIISYETFRL HVGVLQKGSVGLVICDEGHRLKNSE NQTYQALDSLNTSRRVLISGTPIQN DLLEYFSLVHFVNSGILGTA HEFKKHFELPILKGRDAAASEADRQ LGEERLRELTSIVNRCLIRRTSDIL SKYLPVKIEQVVCCRLTPLQ TELYKRFLRQAKPAEELLEGKMSVS SLSSITSLKKLCNHPALIYDKCVEE EDGFVGALDLFPPGYSSKAL EPQLSGKMLVLDYILAVTRSRSSDK VVLVSNYTQTLDLFEKLCRARRYLY VRLDGTMSIKKRAKVVERFN SPSSPDFVFMLSSKAGGCGLNLIGA NRLVMFDPDWNPANDEQAMARVWRD GQKKTCYIYRLLSAGTIEEK IFQRQSHKKALSSCVVDEEQDVERH FSLGELKELFILDEASLSDTHDRLH CRRCVNSRQIRPPPDGSDCT
  SDLAGWNHCTDKWGLRDEVLQAAWD AASTAITFVFHQRSHEEQRGLRTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination

Gene Information

• Gene Name: RAD54L RAD54 like [ Homo sapiens (human) ]
• Official Symbol: RAD54L
• Synonyms: HR54; hHR54; RAD54A; hRAD54
• Gene ID: 8438
• mRNA Refseq: NM_003579.4
• Protein Refseq: NP_003570.2
• MIM: 603615
• UniProt ID: Q92698