Recombinant Full Length Human RASGRF1 Protein, C-Flag-tagged

• Cat.No.: RASGRF1-1138HFL
• Product Overview: Recombinant Full Length Human RASGRF1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 145.1 kDa
• AA Sequence: MQKGIRLNDGHVASLGLLARKDGTR KGYLSKRSSDNTKWQTKWFALLQNL LFYFESDSSSRPSGLYLLEG CVCDRAPSPKPALSAKEPLEKQHYF TVNFSHENQKALELRTEDAKDCDEW VAAIAHASYRTLATEHEALM QKYLHLLQIVETEKTVAKQLRQQIE DGEIEIERLKAEITSLLKDNERIQS TQTVAPNDEDSDIKKIKKVQ SFLRGWLCRRKWKTIIQDYIRSPHA DSMRKRNQVVFSMLEAEAEYVQQLH ILVNNFLRPLRMAASSKKPP ITHDDVSSIFLNSETIMFLHQIFYQ GLKARISSWPTLVLADLFDILLPML NIYQEFVRNHQYSLQILAHC KQNRDFDKLLKHYEAKPDCEERTLE TFLTYPMFQIPRYILTLHELLAHTP HEHVERNSLDYAKSKLEELS RIMHDEVSETENIRKNLAIERMIIE GCEILLDTSQTFVRQGSLIQVPMSE KGKITRGRLGSLSLKKEGER QCFLFSKHLIICTRGSGGKLHLTKN GVISLIDCTLLEEPESTEEEAKGSG QDIDHLDFKIGVEPKDSPPF TVILVASSRQEKAAWTSDISQCVDN IRCNGLMMNAFEENSKVTVPQMIKR TREGTREAEMSRSDASLYCD DVDIRFSKTMNSCKVLQIRYASVER LLERLTDLRFLSIDFLNTFLHSYRV FTTAIVVLDKLITIYKKPIS AIPARWLRSLELLFASGQNNKLLYG EPPKSPRATRKFSSPPPLSITKTSS PSRRRKLSLNIPIITGGKAL DLAALSCNSNGYTSMYSAMSPFSKA TLDTSKLYVSSSFTNKIPDEGDTTP EKPEDPSALSKQSSEVSMRE ESDIDQNQSDDGDTETSPTKSPTTP KSVKNKNSSEFPLFSYNNGVVMTSC RELDNNRSALSAASAFAIAT AGANEGTPNKEKYRRMSLASAGFPP DQRNGDKEFVIRRAATNRVLNVLRH WVSKHSQDFETNDELKCKVI GFLEEVMHDPELLTQERKAAANIIR TLTQEDPGDNQITLEEITQMAEGVK AEPFENHSALEIAEQLTLLD HLVFKKIPYEEFFGQGWMKLEKNER TPYIMKTTKHFNDISNLIASEIIRN EDINARVSAIEKWVAVADIC RCLHNYNAVLEITSSMNRSAIFRLK KTWLKVSKQTKALIDKLQKLVSSEG RFKNLREALKNCDPPCVPYL GMYLTDLAFIEEGTPNYTEDGLVNF SKMRMISHIIREIRQFQQTAYKIEH QAKVTQYLLDQSFVMDEESL
  YESSLRIEPKLPTTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, MAPK signaling pathway

Gene Information

• Gene Name: RASGRF1 Ras protein specific guanine nucleotide releasing factor 1 [ Homo sapiens (human) ]
• Official Symbol: RASGRF1
• Synonyms: GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
• Gene ID: 5923
• mRNA Refseq: NM_002891.6
• Protein Refseq: NP_002882.3
• MIM: 606600
• UniProt ID: Q13972