Recombinant Full Length Human RFC3 Protein, C-Myc/DDK-tagged

• Cat.No.: RFC3-04HFL
• Product Overview: Recombinant Full Length Human RFC3 Protein, fused to Myc/DDK-tag at C-terminus, was expressed in HEK293T.

Specification

• Description: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Form: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Molecular Mass: 40.4 kDa
• AA Sequence: MSLWVDKYRPCSLGRLDYHKEQAAQ LRNLVQCGDFPHLLVYGPSGAGKKT RIMCILRELYGVGVEKLRIE HQTITTPSKKKIEISTIASNYHLEV NPSDAGNSDRVVIQEMLKTVAQSQQ LETNSQRDFKVVLLTEVDKL TKDAQHALRRTMEKYMSTCRLILCC NSTSKVIPPIRSRCLAVRVPAPSIE DICHVLSTVCKKEGLNLPSQ LAHRLAEKSCRNLRKALLMCEACRV QQYPFTADQEIPETDWEVYLRETAN AIVSQQTPQRLLEVRGRLYE LLTHCIPPEIIMKGLLSELLHNCDG QLKGEVAQMAAYYEHRLQLGSKAIY HLEAFVAKFMALYKKFMEDG LEGMMF TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Applications: Cell culture: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair

Gene Information

• Gene Name: RFC3 replication factor C subunit 3 [ Homo sapiens (human) ]
• Official Symbol: RFC3
• Synonyms: RFC38
• Gene ID: 5983
• mRNA Refseq: NM_002915.4
• Protein Refseq: NP_002906.1
• MIM: 600405
• UniProt ID: P40938