Recombinant Full Length Human ROBO2 Protein, C-Flag-tagged

• Cat.No.: ROBO2-674HFL
• Product Overview: Recombinant Full Length Human ROBO2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 152.9 kDa
• AA Sequence: MARRHERVTRRMWTWAPGLLMMTVV FWGHQGNGQGQGSRLRQEDFPPRIV EHPSDVIVSKGEPTTLNCKA EGRPTPTIEWYKDGERVETDKDDPR SHRMLLPSGSLFFLRIVHGRRSKPD EGSYVCVARNYLGEAVSRNA SLEVALLRDDFRQNPTDVVVAAGEP AILECQPPRGHPEPTIYWKKDKVRI DDKEERISIRGGKLMISNTR KSDAGMYTCVGTNMVGERDSDPAEL TVFERPTFLRRPINQVVLEEEAVEF RCQVQGDPQPTVRWKKDDAD LPRGRYDIKDDYTLRIKKTMSTDEG TYMCIAENRVGKMEASATLTVRAPP QFVVRPRDQIVAQGRTVTFP CETKGNPQPAVFWQKEGSQNLLFPN QPQQPNSRCSVSPTGDLTITNIQRS DAGYYICQALTVAGSILAKA QLEVTDVLTDRPPPIILQGPANQTL AVDGTALLKCKATGDPLPVISWLKE GFTFPGRDPRATIQEQGTLQ IKNLRISDTGTYTCVATSSSGETSW SAVLDVTESGATISKNYDLSDLPGP PSKPQVTDVTKNSVTLSWQP GTPGTLPASAYIIEAFSQSVSNSWQ TVANHVKTTLYTVRGLRPNTIYLFM VRAINPQGLSDPSPMSDPVR TQDISPPAQGVDHRQVQKELGDVLV RLHNPVVLTPTTVQVTWTVDRQPQF IQGYRVMYRQTSGLQATSSW QNLDAKVPTERSAVLVNLKKGVTYE IKVRPYFNEFQGMDSESKTVRTTEE APSAPPQSVTVLTVGSYNST SISVSWDPPPPDHQNGIIQEYKIWC LGNETRFHINKTVDAAIRSVIIGGL FPGIQYRVEVAASTSAGVGV KSEPQPIIIGRRNEVVITENNNSIT EQITDVVKQPAFIAGIGGACWVILM GFSIWLYWRRKKRKGLSNYA VTFQRGDGGLMSNGSRPGLLNAGDP SYPWLADSWPATSLPVNNSNSGPNE IGNFGRGDVLPPVPGQGDKT ATMLSDGAIYSSIDFTTKTSYNSSS QITQATPYATTQILHSNSIHELAVD LPDPQWKSSIQQKTDLMGFG YSLPDQNKGNNGGKGGKKKKNKNSS KPQKNNGSTWANVPLPPPPVQPLPG TELEHYAVEQQENGYDSDSW CPPLPVQTYLHQGLEDELEEDDDRV PTPPVRGVASSPAISFGQQSTATLT PSPREEMQPMLQAHLDELTR AYQFDIAKQTWHIQSNNQPPQPPVP PLGYVSGALISDLETDVADDDADDE EEALEIPRPLRALDQTPGSS MDNLDSSVTGKAFTSSQRPRPTSPF STDSNTSAALSQSQRPRPTKKHKGG RMDQQPALPHRREGMTDEEA
  LVPYSKPSFPSPGGHSSSGTASSKG STGPRKTEVLRAGHQRNASDLLDIG YMGSNSQGQFTGELTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance

Gene Information

• Gene Name: ROBO2 roundabout guidance receptor 2 [ Homo sapiens (human) ]
• Official Symbol: ROBO2
• Synonyms: SAX3
• Gene ID: 6092
• mRNA Refseq: NM_001128929.3
• Protein Refseq: NP_001122401.1
• MIM: 602431
• UniProt ID: Q9HCK4