Recombinant Full Length Human SH2B3 Protein, C-Flag-tagged

• Cat.No.: SH2B3-812HFL
• Product Overview: Recombinant Full Length Human SH2B3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 63 kDa
• AA Sequence: MNGPALQPSSPSSAPSASPAAAPRG WSEFCELHAVAAARELARQYWLFAR EHPQHAPLRAELVSLQFTDL FQRYFCREVRDGRAPGRDYRDTGRG PPAKAEASPEPGPGPAAPGLPKARS SEELAPPRPPGPCSFQHFRR SLRHIFRRRSAGELPAAHTAAAPGT PGEAAETPARPGLAKKFLPWSLARE PPPEALKEAVLRYSLADEAS MDSGARWQRGRLALRRAPGPDGPDR VLELFDPPKSSRPKLQAACSSIQEV RRCTRLEMPDNLYTFVLKVK DRTDIIFEVGDEQQLNSWMAELSEC TGRGLESTEAEMHIPSALEPSTSSS PRGSTDSLNQGASPGGLLDP ACQKTDHFLSCYPWFHGPISRVKAA QLVQLQGPDAHGVFLVRQSETRRGE YVLTFNFQGIAKHLRLSLTE RGQCRVQHLHFPSVVDMLHHFQRSP IPLECGAACDVRLSSYVVVVSQPPG SCNTVLFPFSLPHWDSESLP HWGSELGLPHLSSSGCPRGLSPEGL PGRSSPPEQIFHLVPSPEELANSLQ HLEHEPVNRARDSDYEMDSS
  SRSHLRAIDNQYTPLTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Neurotrophin signaling pathway

Gene Information

• Gene Name: SH2B3 SH2B adaptor protein 3 [ Homo sapiens (human) ]
• Official Symbol: SH2B3
• Synonyms: LNK; IDDM20
• Gene ID: 10019
• mRNA Refseq: NM_005475.3
• Protein Refseq: NP_005466.1
• MIM: 605093
• UniProt ID: Q9UQQ2